RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.

中文翻译：

---

TimeLapse-seq：通过核苷重新编码为 RNA 测序添加时间维度

RNA 测序 (RNA-seq) 提供细胞 RNA 群体的快照，但不提供有关已测序 RNA 的时间信息。在这里，我们报告了 TimeLapse-seq，它使用氧化亲核芳香取代将 4-硫尿苷转化为胞苷类似物，产生明显的 U 到 C 突变，在测序时标记新的转录本。 TimeLapse-seq 是一种单分子方法，适用于多种应用，可揭示传统 RNA-seq 中隐藏的 RNA 动力学和诱导差异表达。