To date, most of invasive cell sheet harvesting methods have used culture surface property variations, such as wettability, pH, electricity, and magnetism, to induce cell detachment. These methods that rely on surface property changes are effective when cell detachment prior to application is necessary, but of limited use when used for cell sheet transfer to target regions. The study reports a new reactive oxygen species (ROS)-induced strategy based on hematoporphyrin-incorporated polyketone film (Hp-PK film) to transfer cell sheets directly to target areas without an intermediate harvesting process. After green LED (510 nm) irradiation, production of exogenous ROS from the Hp-PK films induces cell sheet detachment and transfer. The study suggests that ROS-induced cell detachment property of the Hp-PK film is closely related to conformational changes of extracellular matrix (ECM) proteins. Also, this strategy with the Hp-PK film can be applied by regulating production rate of exogenous ROS in various types of cells, including fibroblasts, mesenchymal stem cells and keratinocytes. In conclusion, ROS-induced method using the Hp-PK film can be used for one-step cell sheet transplantation and has potential in biomedical applications.

迄今为止，大多数侵入性细胞片收获方法已经使用了培养物表面性质的变化，例如润湿性，pH，电和磁性，来诱导细胞脱离。当需要在施加细胞之前进行细胞分离时，这些依赖于表面性质变化的方法是有效的，但是当用于将细胞片转移至目标区域时，这些方法的用途有限。这项研究报告了一种新的活性氧（ROS）诱导的策略，该策略基于掺入血卟啉的聚酮薄膜（Hp-PK薄膜），可将细胞片直接转移到目标区域，而无需中间的收获过程。在绿色LED（510 nm）照射后，Hp-PK膜产生外源性ROS诱导细胞片分离和转移。研究表明，ROS诱导的Hp-PK膜的细胞分离特性与细胞外基质（ECM）蛋白的构象变化密切相关。同样，可以通过调节各种类型的细胞（包括成纤维细胞，间充质干细胞和角质形成细胞）中外源性ROS的产生率来应用具有Hp-PK膜的这种策略。总之，使用Hp-PK膜的ROS诱导方法可用于一步细胞片移植，并在生物医学应用中具有潜力。