This work attempted to engineer emulsions’ interface using the special affinity between proline-rich gliadin and proanthocyanidins (PA), to develop surfactant-free antioxidant Pickering emulsions with digestive-resistant properties. This binding interaction between gliadin and PA benefited the interfacial adsorption of the particles to corn oil droplets. Pickering droplets as building units assembled into an interconnected three-dimensional network structure, giving the emulsions viscoelasticity and ultrastability. Oxidative markers in Pickering emulsions were periodically monitored under thermally accelerated storage. Lipid digestion and oxidation fates were characterized using in vitro gastrointestinal (GI) models. The interfacial membrane constructed by antioxidant particles served as a valid barrier against lipid oxidation and digestion, in a PA dose-dependent manner. Briefly, lipid oxidation under storage and simulated GI tract was retarded. Free fatty acid (FFA) fraction released decreased by 55% from 87.9% (bulk oil) to 39.5% (Pickering emulsion), implying engineering interfacial architecture potentially benefited to fight obesity. This study opens a facile strategy to tune lipid oxidation and digestion profiles through the cooperation of the Pickering principle and the interfacial delivery of antioxidants.

中文翻译：

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麦醇溶蛋白/原花青素杂化颗粒（GPHP）稳定的匹克乳化剂的开发以及脂质氧化和消化的命运

这项工作试图利用富含脯氨酸的麦醇溶蛋白和原花青素（PA）之间的特殊亲和力来设计乳液的界面，从而开发出具有抗消化特性的不含表面活性剂的抗氧化剂Pickering乳液。麦醇溶蛋白和PA之间的这种结合相互作用有利于颗粒向玉米油滴的界面吸附。作为建筑单元的Pickering液滴组装成相互连接的三维网络结构，使乳液具有粘弹性和超稳定性。在热加速储存条件下，定期监测Pickering乳液中的氧化标记。使用体外胃肠道（GI）模型表征脂质的消化和氧化命运。由抗氧化剂颗粒构成的界面膜可有效抵抗脂质氧化和消化，以PA剂量依赖性方式。简而言之，在储存和模拟的胃肠道下脂质氧化被延迟。释放的游离脂肪酸（FFA）比例从87.9％（散装油）下降了55％，降至39.5％（Pickering乳液），这表明工程界面结构可能有益于对抗肥胖症。这项研究为通过Pickering原理和抗氧化剂的界面传递协同调节脂质氧化和消化特性提供了一种简便的策略。