To study the effect and underlying molecular mechanism of eugenol on CD11b⁺Gr1⁺ myeloid-derived suppressor cells (MDSCs). The effect of eugenol on the inhibition of immortalized MDSC cell line MSC-2 and murine peritoneal macrophages was detected by MTT. Flow cytometry was used to detect the pro-apoptosis effect of eugenol on MDSCs. The expression levels of apoptosis-related proteins were detected by western blot. Eugenol has a selective inhibitory effect on MDSCs in a dose-dependent manner, which activates an endogenous apoptosis pathway, leading to apoptosis. Eugenol promotes the apoptosis of MDSCs via the intrinsic pathway.

中文翻译：

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丁香酚通过内源性凋亡通路触发 CD11b+Gr1+ 髓源性抑制细胞凋亡

研究丁香酚对 CD11b ⁺ Gr1 ⁺髓源性抑制细胞 (MDSCs) 的影响及其潜在分子机制。MTT法检测丁香酚对永生化MDSC细胞系MSC-2和小鼠腹腔巨噬细胞的抑制作用。流式细胞仪检测丁香酚对MDSCs的促凋亡作用。Western blot检测凋亡相关蛋白的表达水平。丁香酚对 MDSCs 具有剂量依赖性的选择性抑制作用，可激活内源性凋亡通路，导致细胞凋亡。丁香酚通过内在途径促进 MDSCs 的凋亡。