Magnetic Macroporous Hydrogels as a Novel Approach for Perfused Stem Cell Culture in 3D Scaffolds via Contactless Motion Control,Advanced Healthcare Materials

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Magnetic Macroporous Hydrogels as a Novel Approach for Perfused Stem Cell Culture in 3D Scaffolds via Contactless Motion Control
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-01-19 , DOI: 10.1002/adhm.201701403
Lisa Rödling ₁ , Esther Magano Volz ₁ , Annamarija Raic ₁ , Katharina Brändle ₁ , Matthias Franzreb ₁ , Cornelia Lee-Thedieck ₁

Affiliation

There is an urgent need for 3D cell culture systems that avoid the oversimplifications and artifacts of conventional culture in 2D. However, 3D culture within the cavities of porous biomaterials or large 3D structures harboring high cell numbers is limited by the needs to nurture cells and to remove growth‐limiting metabolites. To overcome the diffusion‐limited transport of such soluble factors in 3D culture, mixing can be improved by pumping, stirring or shaking, but this in turn can lead to other problems. Using pumps typically requires custom‐made accessories that are not compatible with conventional cell culture disposables, thus interfering with cell production processes. Stirring or shaking allows little control over movement of scaffolds in media. To overcome these limitations, magnetic, macroporous hydrogels that can be moved or positioned within media in conventional cell culture tubes in a contactless manner are presented. The cytocompatibility of the developed biomaterial and the applied magnetic fields are verified for human hematopoietic stem and progenitor cells (HSPCs). The potential of this technique for perfusing 3D cultures is demonstrated in a proof‐of‐principle study that shows that controlled contactless movement of cell‐laden magnetic hydrogels in culture media can mimic the natural influence of differently perfused environments on HSPCs.

中文翻译：

磁性大孔水凝胶作为通过非接触运动控制在3D支架中灌注干细胞培养的新方法

迫切需要能够避免2D常规培养的过度简化和伪影的3D细胞培养系统。但是，在多孔生物材料或具有大量细胞的大型3D结构的腔体内进行3D培养受到培育细胞和去除生长受限代谢物的需求的限制。为了克服此类可溶性因子在3D培养物中的扩散限制运输，可以通过泵送，搅拌或摇晃来改善混合效果，但这又会导致其他问题。使用泵通常需要定制配件，这些配件与常规的细胞培养一次性用品不兼容，从而干扰了细胞生产过程。搅拌或摇动几乎不能控制支架在介质中的运动。为了克服这些限制，磁性，提出了可以非接触方式在常规细胞培养管中的培养基中移动或定位的大孔水凝胶。对于人类造血干细胞和祖细胞（HSPC），已开发的生物材料的细胞相容性和所施加的磁场得到了验证。一项原理研究证明了该技术在3D培养物中的潜能，该研究表明，载有培养基的细胞内磁水凝胶的受控非接触运动可以模仿不同灌注环境对HSPC的自然影响。

更新日期：2018-01-19

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