Humulus lupulus L. (hop) represents one of the most cultivated crops, it being a key ingredient in the brewing process. Many health-related properties have been described for hop extracts, making this plant gain more interest in the field of pharmaceutical and nutraceutical research. Among the analytical tools available for the phytochemical characterization of plant extracts, quantitative nuclear magnetic resonance (qNMR) represents a new and powerful technique. In this ambit, the present study was aimed at the development of a new, simple, and efficient qNMR method for the metabolite fingerprinting of bioactive compounds in hop cones, taking advantage of the novel ERETIC 2 tool. To the best of our knowledge, this is the first attempt to apply this method to complex matrices of natural origin, such as hop extracts. The qNMR method set up in this study was applied to the quantification of both prenylflavonoids and bitter acids in eight hop cultivars. The performance of this analytical method was compared with that of HPLC-UV/DAD, which represents the most frequently used technique in the field of natural product analysis. The quantitative data obtained for hop samples by means of the two aforementioned techniques highlighted that the amount of bioactive compounds was slightly higher when qNMR was applied, although the order of magnitude of the values was the same. The accuracy of qNMR was comparable to that of the chromatographic method, thus proving to be a reliable tool for the analysis of these secondary metabolites in hop extracts.

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啤酒花大号。（啤酒花）代表了耕种最多的农作物之一，它是酿造过程中的关键成分。酒花提取物已经描述了许多与健康有关的特性，这使得该植物在制药和营养保健研究领域越来越受到关注。在可用于植物提取物植物化学表征的分析工具中，定量核磁共振（qNMR）代表了一种新的强大技术。在此范围内，本研究旨在利用新型ERETIC 2工具开发一种新的，简单而有效的qNMR方法，用于啤酒花球果中生物活性化合物的代谢物指纹图谱。据我们所知，这是将这种方法应用于天然来源的复杂基质（例如啤酒花提取物）的首次尝试。在这项研究中建立的qNMR方法用于量化八种啤酒花中异戊二烯类黄酮和苦味酸的含量。将该分析方法的性能与HPLC-UV / DAD的性能进行了比较，后者代表了天然产物分析领域中最常用的技术。通过上述两种技术获得的蛇麻草样品的定量数据突出表明，尽管应用了qNMR，但生物活性化合物的含量略高，尽管数值的数量级相同。qNMR的准确性与色谱法相当，因此被证明是分析啤酒花提取物中这些次生代谢产物的可靠工具。将该分析方法的性能与HPLC-UV / DAD的性能进行了比较，后者代表了天然产物分析领域中最常用的技术。通过上述两种技术获得的蛇麻草样品的定量数据突出表明，尽管应用了qNMR，但生物活性化合物的含量略高，尽管数值的数量级相同。qNMR的准确性与色谱法相当，因此被证明是分析啤酒花提取物中这些次生代谢产物的可靠工具。将该分析方法的性能与HPLC-UV / DAD的性能进行了比较，后者代表了天然产物分析领域中最常用的技术。通过上述两种技术获得的蛇麻草样品的定量数据突出表明，尽管应用了qNMR，但生物活性化合物的含量略高，尽管数值的数量级相同。qNMR的准确性与色谱法相当，因此被证明是分析啤酒花提取物中这些次生代谢产物的可靠工具。通过上述两种技术获得的蛇麻草样品的定量数据突出表明，尽管应用了qNMR，但生物活性化合物的含量略高，尽管数值的数量级相同。qNMR的准确性与色谱法相当，因此被证明是分析啤酒花提取物中这些次生代谢产物的可靠工具。通过上述两种技术获得的蛇麻草样品的定量数据突出表明，尽管应用了qNMR，但生物活性化合物的含量略高，尽管数值的数量级相同。qNMR的准确性与色谱法相当，因此被证明是分析啤酒花提取物中这些次生代谢产物的可靠工具。

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