Functionalization of microbubbles (MBs) is a difficult issue due to their unstable nature. Here we report a fast and versatile method using a strain promoted alkyne–azide cycloaddition (SPAAC) click reaction for microbubble functionalization. An azadibenzocyclooctyne (DBCO) group was first introduced onto the MB surface and then an azide group into the desired ligand. Without any initiators or catalysts, essential click ligation occurred within 1 min and a majority of the reaction completed in 5 min at 37 °C. This fast ligation shortens the microbubble reaction time and preserves essential amounts of microbubbles for further in situ imaging and delivery of therapeutics.

中文翻译：

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使用应变促进点击化学快速功能化超声微泡

由于其不稳定的性质，微泡（MB）的功能化是一个难题。在这里，我们报告了一种使用应变促进的炔叠氮环加成（SPAAC）点击反应进行微泡功能化的快速且通用的方法。首先将氮杂二苯并环辛炔 (DBCO) 基团引入 MB 表面，然后将叠氮基团引入所需的配体中。在没有任何引发剂或催化剂的情况下，必要的点击连接在 1 分钟内发生，大部分反应在 37 °C 下于 5 分钟内完成。这种快速连接缩短了微泡反应时间，并保留了必要量的微泡，用于进一步的原位成像和治疗药物的递送。