Arthrobacter sp. CGMCC 3584 is able to produce high yields of extracellular cyclic adenosine monophosphate (cAMP), which plays a vital role in the field of treatment of disease and animal food, during aerobic fermentation. However, the molecular basis of cAMP production in Arthrobacter species is rarely explored. Here, for the first time, we report the comparative transcriptomic and proteomic study of Arthrobacter cells to elucidate the higher productivity of cAMP under high oxygen supply. We finally obtained 14.1% and 19.3% of the Arthrobacter genome genes which were up-regulated and down-regulated notably, respectively, with high oxygen supply, and identified 54 differently expressed proteins. Our results revealed that high oxygen supply had two major effects on metabolism: inhibition of glycolysis, pyruvate metabolism, nitrogen metabolism, and amino acid metabolism (histidine, branched-chain amino acids and glutamate metabolism); enhancement of the tricarboxylic acid cycle and purine metabolism. We also found that regulation of adenylate cyclase and phosphodiesterase was not significant under high oxygen supply, suggesting efficient cAMP export might be important in cAMP production. These findings may contribute to further understanding of capacities of Arthrobacter species and would be highly useful in genetic regulation for desirable production.

中文翻译：

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关节杆菌属的比较转录组学和蛋白质组学分析。CGMCC 3584对溶解氧作出响应，以产生cAMP。

关节杆菌 CGMCC 3584能够产生高产量的细胞外环状单磷酸腺苷（cAMP），在需氧发酵过程中，它在疾病和动物食品的治疗领域中起着至关重要的作用。但是，在节杆菌物种中产生cAMP的分子基础很少被探索。在这里，我们首次报道了节杆菌细胞的比较转录组学和蛋白质组学研究，以阐明在高氧供应下cAMP的更高生产率。我们最终获得了节肢杆菌基因组基因的14.1％和19.3％，它们分别在高氧气供应下显着上调和下调，并鉴定了54种不同表达的蛋白。我们的结果表明，高氧供应对代谢有两个主要影响：抑制糖酵解，丙酮酸代谢，氮代谢，和氨基酸代谢（组氨酸，支链氨基酸和谷氨酸代谢）；增强三羧酸循环和嘌呤代谢。我们还发现，在高氧气供应下，腺苷酸环化酶和磷酸二酯酶的调控并不重要，这表明有效的cAMP出口可能对cAMP的生产很重要。这些发现可能有助于进一步了解节杆菌属物种的能力，并且在遗传调控中对所需产量具有非常大的帮助。提示有效的cAMP出口可能对cAMP生产很重要。这些发现可能有助于进一步了解节杆菌属物种的能力，并且在遗传调控中对所需产量具有非常大的帮助。提示有效的cAMP出口可能对cAMP生产很重要。这些发现可能有助于进一步了解节杆菌属物种的能力，并且在遗传调控中对所需产量具有非常大的帮助。