Accurate characterization of the mechanical properties of brain tissue is essential for understanding the mechanisms of traumatic brain injuries and developing protective gears or facilities. However, how storage conditions might affect the mechanical properties of brain tissue remains unclear. The objective of this study is to investigate the effect of in vitro storage duration on the mechanical performance of brain tissue since measurements are usually carried out in vitro. Differential Scanning Calorimetry (DSC) measurements and uniaxial compression mechanical experiments are carried out. The results indicate that, for brain tissue stored at 1 °C without any liquid medium, the bio-molecular interactions and the mechanical strength of both white and grey matter deteriorate with prolonged storage duration. Transmission Electron Microscopy (TEM) results reveal the degeneration of myelin sheaths and the vacuolization of cristae with prolonged storage duration, suggesting that the in vitro storage duration should be carefully controlled. The findings from this study might facilitate the development of guidelines and standards for the in vitro storage of brain tissue.

中文翻译：

---

体外储存时间对测得的脑组织机械性能的影响。

准确表征大脑组织的机械特性对于理解颅脑外伤的机制和开发防护装备或设施至关重要。然而，尚不清楚储存条件如何影响脑组织的机械性能。这项研究的目的是研究体外保存时间对大脑组织机械性能的影响，因为测量通常是在体外进行的。进行了差示扫描量热法（DSC）测量和单轴压缩力学实验。结果表明，对于在1°C下没有任何液体介质存储的脑组织，白质和灰质的生物分子相互作用和机械强度会随着存储时间的延长而恶化。透射电子显微镜（TEM）结果显示髓鞘的变性和ista的空泡化具有较长的储存时间，这表明应仔细控制体外储存时间。这项研究的发现可能有助于脑组织体外储存的指导方针和标准的发展。