Effects of Plant Sterols or β-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death,Journal of Agricultural and Food Chemistry

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Effects of Plant Sterols or β-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-27 00:00:00 , DOI: 10.1021/acs.jafc.7b05575
Andrea Alvarez-Sala ₁ , Gabriel López-García ₁ , Alessandro Attanzio ₂ , Luisa Tesoriere ₂ , Antonio Cilla ₁ , Reyes Barberá ₁ , Amparo Alegría ₁

Affiliation

The eryptotic and hemolytic effects of a phytosterol (PS) mixture (β-sitosterol, campesterol, stigmasterol) or β-cryptoxanthin (β-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 μM) were evaluated. β-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca²⁺ influx. Co-incubation of both bioactive compounds protected against β-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca²⁺ influx, ROS overproduction and GSH depletion at 75 μM, and hemolysis at both tBOOH concentrations. β-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by β-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.

中文翻译：

血清浓度对植物甾醇或β-隐黄质的影响对自杀性红细胞死亡的影响

植物甾醇（PS）混合物（β-谷固醇，菜油甾醇，豆甾醇）或β-隐黄质（β-Cx）在生理性血清浓度下的隐性和溶血作用及其对叔丁基过氧化氢（tBOOH）诱导的氧化应激的作用（75和300μM）进行评估。β-隐黄质增加了隐性细胞，细胞体积，溶血和谷胱甘肽耗竭（GSH）的增加，而没有ROS过量产生和细胞内Ca ²⁺大量涌入。两种生物活性化合物的共温育可防止β-Cx诱导的隐匿。在tBOOH胁迫下，PS阻止了加密，减少了Ca ²⁺两种tBOOH浓度下均会出现大量涌入，ROS过度产生和GSH消耗以及两种BOOOH浓度的溶血现象。β-隐黄质没有细胞保护作用。与两种生物活性化合物共同孵育可以完全防止溶血，部分可以防止由β-Cx和tBOOH引起的隐性分泌以及GSH消耗。生理血清浓度的植物甾醇有助于预防促隐和溶血作用，并且是有望改善与隐匿性疾病相关的疾病的候选化合物。

更新日期：2018-01-27

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