In this issue of Blood , Legut et al have tackled a major scientific challenge relating to T-cell engineering, with an unexpected outcome. They have demonstrated that CRISPR-mediated knockout of the native T-cell receptor (TCR) β chain dramatically improves the expression of inserted therapeutic αβ and γδ TCRs in comparison with the same TCRs inserted without endogenous TCR-β chain knockout. Although insertion of TCRs or chimeric antigen receptors (CARs) into the TCR locus has been reported previously, the significant improvement in functional avidity of TCR modified T cells shown here is novel. 1

中文翻译：

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编辑基因工程以增强功能

在本期血液中，Legut 等人解决了与 T 细胞工程相关的重大科学挑战，并取得了意想不到的结果。他们已经证明，与没有内源性 TCR-β 链敲除的相同 TCR 插入相比，CRISPR 介导的天然 T 细胞受体 (TCR) β 链敲除显着提高了插入的治疗性 αβ 和 γδ TCR 的表达。尽管之前已经报道了将 TCR 或嵌合抗原受体 (CAR) 插入 TCR 位点，但此处显示的 TCR 修饰的 T 细胞功能亲和力的显着改善是新颖的。1