Abstract Worldwide appreciated beverage, “Arabica” and “Robusta” coffee produced from Coffea arabica and C. canephora respectively, represents a high economical value product. Arabica coffee is generally sold at a higher price than Robusta coffee. As both intentional and accidental adulterations of Arabica with Robusta are frequent, a reliable, inexpensive, and high-throughput adulteration detection technology is required for the coffee industry. We developed a DNA-based authentication method that overcomes limitations of metabolic profiling of Robusta and Arabica coffees. The identification of Arabica and Robusta coffee species, as well as the quantification of their relative proportion in blends were performed by High Resolution Melting (HRM) analysis on green and roasted coffee products. For a more sensitive detection method, chloroplastic rather than nuclear genetic variations were targeted between Coffea species. By the developed method, the Coffea species of origin can be quickly differentiated and in case of adulteration, the percentage can be determined.

中文翻译：

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开发一种快速有效的基于 DNA 的方法来检测和量化咖啡中的掺假（阿拉比卡咖啡与罗布斯塔咖啡）

摘要 世界知名的饮料，分别由小粒咖啡和中叶咖啡生产的“阿拉​​比卡”和“罗布斯塔”咖啡是一种高经济价值的产品。阿拉比卡咖啡的售价通常高于罗布斯塔咖啡。由于阿拉比卡咖啡与罗布斯塔咖啡的有意和无意掺假很常见，因此咖啡行业需要一种可靠、廉价且高通量的掺假检测技术。我们开发了一种基于 DNA 的认证方法，克服了罗布斯塔咖啡和阿拉比卡咖啡代谢分析的局限性。阿拉比卡和罗布斯塔咖啡种类的鉴定，以及它们在混合物中的相对比例的量化是通过对生咖啡和烘焙咖啡产品的高分辨率熔化 (HRM) 分析进行的。对于更灵敏的检测方法，叶绿体而不是核遗传变异是咖啡物种之间的目标。通过开发的方法，可以快速区分咖啡豆的原产地种类，并且在掺假的情况下，可以确定百分比。