Bio-based production of succinic acid (SA) is regarded to supplement the petro-based processing for environmental reasons. However, in order to enhance the industrial competitiveness of bio-based SA, it is necessary to develop a method that is cost-effective, environmental friendly and with high efficiency. In this study, the utilization of fruit and vegetable waste (FVW) hydrolysate for SA production via engineered Yarrowia lipolytica was reported for the first time. In the study, the hydrolysis conditions were firstly optimized and the final glucose concentration of 56.7 g L⁻¹ and a yield of 0.46 g g⁻¹ were obtained with 2% glucoamylase, 1% cellulase, 2% hemicellulase and 0.25% pectinase at pH 5.0 and 55 °C. Then, the feasibility of using the hydrolysate for SA production via Y. lipolytica PSA02004 was demonstrated. SA titer and yield at 43.1 g L⁻¹ and 0.46 g g⁻¹, respectively were obtained using 100 g L⁻¹ glucose-containing FVW hydrolysate and 4% corn steep liquor (CSL) as fermentation medium by free cell batch fermentation. Finally, in situ fibrous bed bioreactor (isFBB) and fed batch fermentation were demonstrated to be effective approach for improved SA production, from which SA productivity of 0.69 g L⁻¹ h⁻¹ and the resultant SA concentration of 140.6 g L⁻¹ were achieved, respectively. In summary, this study achieved the anticipated target of turning waste into value-added products with high efficiency and cost-effectiveness.

出于环境原因，琥珀酸（SA）的生物基生产被认为是对石油基加工的补充。然而，为了增强生物基SA的工业竞争力，有必要开发一种经济高效，环保且高效的方法。在这项研究中，首次报道了通过工程化解脂耶氏酵母（Yarrowia lipolytica）利用果蔬废料（FVW）水解产物生产SA 。在研究中，首先优化了水解条件，最终葡萄糖浓度为56.7 g L ^-1，产量为0.46 g g ^-1在pH 5.0和55°C下，分别用2％的葡糖淀粉酶，1％的纤维素酶，2％的半纤维素酶和0.25％的果胶酶制得淀粉。然后，证明了将水解产物用于通过解脂耶氏酵母PSA02004生产SA的可行性。使用100 g L ^-1葡萄糖的FVW水解产物和4％玉米浆（CSL）作为发酵培养基，通过游离细胞分批发酵分别获得43.1 g L ^-1和0.46 g g ^-1的SA效价。最后，在原位纤维床生物反应器（是FBB）和补料分批发酵被证明是用于提高SA生产有效的办法，0.69克L-从中SA生产率^-1 ħ ^-1得到的SA浓度分别为140.6g L ^-1。总而言之，这项研究实现了将废物转化为具有高效率和成本效益的增值产品的预期目标。