Separation/Concentration‐signal‐amplification in‐One Method Based on Electrochemical Conversion of Magnetic Nanoparticles for Electrochemical Biosensing,Electroanalysis

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Separation/Concentration‐signal‐amplification in‐One Method Based on Electrochemical Conversion of Magnetic Nanoparticles for Electrochemical Biosensing
Electroanalysis ( IF 2.7 ) Pub Date : 2018-01-18 , DOI: 10.1002/elan.201700653
Lulu Cao ₁ , Qi Zhang ₁ , Huang Dai ₁ , Yingchun Fu ₁ , Yanbin Li _{1,

2}

Affiliation

We propose a separation/concentration‐signal‐amplification in‐one method based on electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to develop a facile and sensitive electrochemical biosensor for chloramphenicol (CAP) detection. Briefly, aptamer‐modified magnetic nanoparticles (MNPs‐Apt) was designed to capture CAP in sample, then the MNPs‐Apt composite was conjugated to Au electrode through the DNA hybridization between the unoccupied aptamer and a strand of complementary DNA. The ECC method was applied to transfer MNPs labels to electrochemically active Prussian blue (PB). The anodic and cathodic currents of PB were taken for signal readout. Comparing with conventional methods that require electrochemically active labels and related sophisticated labelling procedures, this method explored and integrated the magnetic and electrochemical properties of MNPs into one system, in turn realized magnetic capturing of CAP and signal generation without any additional conventional labels. Taking advantages of the high abundance of iron content in MNPs and the refreshing effect deriving from ECC process, the method significantly promoted the signal amplification. Therefore, the proposed biosensors exhibited linear detection range from 1 to 1000 ng mL⁻¹ and a limit of detection down to 1 ng mL⁻¹, which was better than or comparable with those of most analogues, as well as satisfactory specificity, storage stability and feasibility for real samples. The developed method may lead to new concept for rapid and facile biosensing in food safety, clinic diagnose/therapy and environmental monitoring fields.

中文翻译：

基于磁性纳米粒子电化学转化的分离/富集-信号放大-电化学生物传感

我们提出了一种基于磁性纳米粒子（MNP）的电化学转化（ECC）的分离/浓度信号放大一体化方法，以开发一种用于氯霉素（CAP）检测的灵敏，灵敏的电化学生物传感器。简而言之，设计适配体修饰的磁性纳米颗粒（MNPs-Apt）以捕获样品中的CAP，然后通过空置的适配体与互补DNA链之间的DNA杂交将MNPs-Apt复合物偶联到Au电极上。ECC方法用于将MNPs标记转移到电化学活性的普鲁士蓝（PB）。PB的阳极和阴极电流用于信号读取。与需要电化学活性标记和相关复杂标记程序的常规方法相比，该方法探索了MNP的磁性和电化学性质，并将其整合到一个系统中，从而无需任何其他常规标记即可实现CAP的磁性捕获和信号生成。该方法利用MNP中铁含量高和ECC工艺产生的刷新效果，极大地促进了信号放大。因此，提出的生物传感器的线性检测范围为1至1000 ng mL^-1和最低1 ng mL ^-1的检出限，优于或与大多数类似物相当，并且对真实样品具有令人满意的特异性，储存稳定性和可行性。所开发的方法可能会导致在食品安全，临床诊断/治疗和环境监测领域中快速，便捷地进行生物传感的新概念。

更新日期：2018-01-18

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