Utilization of Sweet Sorghum Juice for the Production of Astaxanthin as a Biorefinery Co-Product by Phaffia rhodozyma,ACS Sustainable Chemistry & Engineering

当前位置： X-MOL 学术 › ACS Sustain. Chem. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Utilization of Sweet Sorghum Juice for the Production of Astaxanthin as a Biorefinery Co-Product by Phaffia rhodozyma
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1021/acssuschemeng.7b03154
Ryan J. Stoklosa ₁ , David B. Johnston ₁ , Nhuan P. Nghiem ₁

Affiliation

This work investigates cultivating the red-pigmented yeast Phaffia rhodozyma in sweet sorghum juice (SSJ) to assess the production of astaxanthin as a potential biorefinery co-product. Shake flask cultures on defined sugar medium indicated that all three sugars (sucrose, glucose, and fructose) could be consumed with adequate nitrogen and nutrient supplementation. Only modest biomass growth and astaxanthin production could be achieved in SSJ without nitrogen supplementation; however, combining nitrogen supplementation with yeast extract in diluted SSJ could metabolize all sugars present in 168 h. A 2 L bioreactor trial with full strength (i.e., undiluted) SSJ produced up to 29 g/L of biomass, 65.4 mg/L of astaxanthin, an overall cell astaxanthin content of 2.49 mg astaxanthin/g dry cell mass, and a volumetric astaxanthin productivity of 0.389 mg/L/h after 168 h of cultivation. Further process optimization is needed since glucose metabolism was incomplete in undiluted SSJ.

中文翻译：

利用甜高粱果汁生产虾青素作为红发夫夫酵母的生物精炼副产品

这项工作研究了红色色素酵母红发夫酵母的培养在甜高粱汁（SSJ）中评估虾青素的产量，将其作为潜在的生物精炼副产品。在确定的糖培养基上摇瓶培养表明，所有三种糖（蔗糖，葡萄糖和果糖）可以通过补充充足的氮和营养素而被消耗。不添加氮，SSJ仅能实现适度的生物量增长和虾青素生产。但是，在稀释的SSJ中将氮补充与酵母提取物结合可以使168小时内存在的所有糖代谢。一项具有全强度（即未稀释）SSJ的2 L生物反应器试验产生了高达29 g / L的生物量，65.4 mg / L的虾青素，总细胞虾青素含量为2.49 mg虾青素/ g干细胞质量以及体积的虾青素培养168小时后的生产率为0.389 mg / L / h。

更新日期：2018-01-17

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11