Glycan-binding proteins (GBPs) play critical roles in diverse cellular functions such as cell adhesion, signal transduction and immune response. Studies of the interaction between GBPs and glycans have been hampered by the availability of high throughput and high-content technologies. Here we report multiplex glycan bead array (MGBA) that allows simultaneous analyses of 384 samples and up to 500 glycans in a single assay. The specificity, sensitivity and reproducibility of MGBA are evaluated using 39 plant lectins, 13 recombinant anti-glycan antibodies, and mammalian GBPs. We demonstrate the utility of this platform by the analyses of natural anti-glycan IgM and IgG antibodies in 961 human serum samples and the discovery of anti-glycan antibody biomarkers for ovarian cancer. Our data indicate that the MGBA platform is particularly suited for large population-based studies that require the analyses of large numbers of samples and glycans.

中文翻译：

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多重聚糖珠阵列用于聚糖结合蛋白的高通量和高内涵分析。


聚糖结合蛋白（GBP）在细胞粘附、信号转导和免疫反应等多种细胞功能中发挥着关键作用。高通量和高含量技术的可用性阻碍了 GBP 和聚糖之间相互作用的研究。在这里，我们报告了多重聚糖珠阵列 (MGBA)，它允许在一次测定中同时分析 384 个样品和多达 500 个聚糖。使用 39 种植物凝集素、13 种重组抗聚糖抗体和哺乳动物 GBP 评估 MGBA 的特异性、敏感性和重现性。我们通过分析 961 份人血清样本中的天然抗聚糖 IgM 和 IgG 抗体以及发现卵巢癌抗聚糖抗体生物标志物来证明该平台的实用性。我们的数据表明，MGBA 平台特别适合需要分析大量样品和聚糖的大规模人群研究。