Silver nanomaterials have been mainly developed as antibacterial healthcare products worldwide, because of their antibacterial activity. However, there is little data regarding the potential risks and effects of large amounts of silver nanomaterials on plants. In contrast, N-glycans play important roles in various biological phenomena, and their structures and expressions are sensitive to ambient environmental changes. Therefore, to assesse the effects of silver nanomaterials, we focused on the correlation between N-glycans and the effects of silver nanomaterials in plants and analyzed N-glycan structures in Oryza sativa seedlings exposed to silver nanocolloids (SNCs). The phenotype analysis showed that the shoot was not affected by any SNC concentrations, whereas the high SNC exposed root was seriously damaged. Therefore, we performed comparative N-glycan analysis of roots. As a result, five of total N-glycans were significantly increased in SNC exposed roots, of which one was a free-N-glycan with one beta-N-acetylglucosamine residue at the reducing end. Our results suggest that the transition of plant complex type N-glycans, including free-N-glycans, was caused by abnormalities in O. sativa development, and free-N-glycan itself has an important role in plant development. This study originally adapted glycome transition analysis to environmental toxicology and proposed a new category called "Environmental glycobiology".

中文翻译：

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银纳米胶体对水稻根系植物复合型N-聚糖的影响。

银纳米材料由于其抗菌活性而已被主要开发为全世界的抗菌保健产品。然而，关于大量银纳米材料对植物的潜在风险和影响的数据很少。相反，N-聚糖在各种生物现象中起重要作用，其结构和表达对周围环境变化敏感。因此，为了评估银纳米材料的作用，我们着重研究了N-聚糖与植物中银纳米材料的作用之间的相关性，并分析了暴露于银纳米胶体（SNC）的水稻幼苗中的N-聚糖结构。表型分析表明，芽不受任何SNC浓度的影响，而高SNC暴露的根严重受损。所以，我们对根进行了N-聚糖比较分析。结果，在暴露于SNC的根中，总共有5种N-聚糖显着增加，其中一种是游离N-聚糖，在还原端带有一个β-N-乙酰氨基葡糖残基。我们的结果表明，植物复合型N-聚糖（包括游离N-聚糖）的转变是由O. sativa发育异常引起的，游离N-聚糖本身在植物发育中具有重要作用。这项研究最初使糖基转移分析适合于环境毒理学，并提出了一个新的类别，称为“环境糖生物学”。我们的结果表明，植物复合型N-聚糖（包括游离N-聚糖）的转变是由O. sativa发育异常引起的，游离N-聚糖本身在植物发育中具有重要作用。这项研究最初使糖基转移分析适合于环境毒理学，并提出了一个新的类别，称为“环境糖生物学”。我们的结果表明，植物复合型N-聚糖（包括游离N-聚糖）的转变是由O. sativa发育异常引起的，游离N-聚糖本身在植物发育中具有重要作用。这项研究最初使糖基转移分析适合于环境毒理学，并提出了一个新的类别，称为“环境糖生物学”。