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Electronic Properties of Synthetic Shrimp Pathogens-derived DNA Schottky Diodes.
Scientific Reports ( IF 3.8 ) Pub Date : 2018-01-17 , DOI: 10.1038/s41598-017-18825-6
Nastaran Rizan , Chan Yen Yew , Maryam Rajabpour Niknam , Jegenathan Krishnasamy , Subha Bhassu , Goh Zee Hong , Sridevi Devadas , Mohamed Shariff Mohd Din , Hairul Anuar Tajuddin , Rofina Yasmin Othman , Siew Moi Phang , Mitsumasa Iwamoto , Vengadesh Periasamy

The exciting discovery of the semiconducting-like properties of deoxyribonucleic acid (DNA) and its potential applications in molecular genetics and diagnostics in recent times has resulted in a paradigm shift in biophysics research. Recent studies in our laboratory provide a platform towards detecting charge transfer mechanism and understanding the electronic properties of DNA based on the sequence-specific electronic response, which can be applied as an alternative to identify or detect DNA. In this study, we demonstrate a novel method for identification of DNA from different shrimp viruses and bacteria using electronic properties of DNA obtained from both negative and positive bias regions in current-voltage (I-V) profiles. Characteristic electronic properties were calculated and used for quantification and further understanding in the identification process. Aquaculture in shrimp industry is a fast-growing food sector throughout the world. However, shrimp culture in many Asian countries faced a huge economic loss due to disease outbreaks. Scientists have been using specific established methods for detecting shrimp infection, but those methods do have their significant drawbacks due to many inherent factors. As such, we believe that this simple, rapid, sensitive and cost-effective tool can be used for detection and identification of DNA from different shrimp viruses and bacteria.

中文翻译:

合成虾病原体衍生的DNA肖特基二极管的电子特性。

近年来,关于脱氧核糖核酸(DNA)的类半导体性质的激动人心的发现及其在分子遗传学和诊断学中的潜在应用,导致了生物物理学研究的范式转变。我们实验室中的最新研究提供了一个平台,可用于检测电荷转移机制并基于序列特异性电子响应了解DNA的电子特性,该平台可作为鉴定或检测DNA的替代方法。在这项研究中,我们展示了一种利用从电流-电压(IV)曲线的负偏压和正偏压区域获得的DNA的电子特性来鉴定不同虾类病毒和细菌DNA的新方法。计算了特征电子性质,并将其用于定量和进一步识别过程中的理解。虾类产业中的水产养殖是世界范围内发展迅速的食品部门。但是,由于疾病暴发,许多亚洲国家的虾类养殖面临巨大的经济损失。科学家们一直在使用特定的既定方法来检测虾类感染,但是由于许多固有因素,这些方法确实存在明显的缺陷。因此,我们认为,这种简单,快速,灵敏且具有成本效益的工具可用于检测和鉴定来自不同虾类病毒和细菌的DNA。由于疾病暴发,许多亚洲国家的虾类养殖面临巨大的经济损失。科学家们一直在使用特定的既定方法来检测虾类感染,但是由于许多固有因素,这些方法确实存在明显的缺陷。因此,我们认为,这种简单,快速,灵敏且具有成本效益的工具可用于检测和鉴定来自不同虾类病毒和细菌的DNA。由于疾病暴发,许多亚洲国家的虾类养殖面临巨大的经济损失。科学家们一直在使用特定的既定方法来检测虾类感染,但是由于许多固有因素,这些方法确实存在明显的缺陷。因此,我们认为,这种简单,快速,灵敏且具有成本效益的工具可用于检测和鉴定来自不同虾类病毒和细菌的DNA。
更新日期:2018-01-17
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