Fluorescence Redox Blinking Adaptable to Structural Analysis of Nucleic Acids,Chemistry - A European Journal

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Fluorescence Redox Blinking Adaptable to Structural Analysis of Nucleic Acids
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2018-03-07 , DOI: 10.1002/chem.201705668
Takafumi Miyata ₁ , Naohiko Shimada ₁ , Atsushi Maruyama ₁ , Kiyohiko Kawai ₂

Affiliation

The phenomenon of blinking is unique to single‐molecule fluorescence measurements. By designing a fluorophore with an appropriate dark‐state lifetime τ_off, a kinetic analysis based on the control of fluorescence blinking (KACB) was devised to investigate the dynamics of biomolecules. By controlling the redox‐reaction‐based blinking (rKACB), conformational dynamics of RNA at the single‐molecule level was previously investigated. However, there is little knowledge about suitable fluorescent molecules for rKACB, and the application of rKACB has been limited to the analysis of hairpins and duplex structures of nucleic acids. In this work, various fluorescent molecules, including Alexa 488, R6G, TAMRA, ATTO 647N and ATTO 655, were evaluated for rKACB. Moreover, rKACB was adapted to the discrimination of DNA/DNA and DNA/RNA nucleic acid duplexes and investigation of antigen–antibody interactions. By changing the size of the oxidant, it was possible to determine the solvent accessibility of the target domain of the analyzed biomolecules.

中文翻译：

荧光氧化还原闪烁适用于核酸的结构分析

闪烁现象是单分子荧光测量所特有的。通过用适当的暗态寿命设计荧光团τ_关，设计了一种基于荧光闪烁（KACB）控制的动力学分析，以研究生物分子的动力学。通过控制基于氧化还原反应的闪烁（rKACB），以前已经研究了RNA在单分子水平上的构象动力学。然而，关于适用于rKACB的荧光分子的知识很少，并且rKACB的应用已经限于发夹和核酸双链体结构的分析。在这项工作中，评估了包括Alexa 488，R6G，TAMRA，ATTO 647N和ATTO 655在内的各种荧光分子的rKACB。此外，rKACB适用于区分DNA / DNA和DNA / RNA核酸双链体以及研究抗原-抗体相互作用。通过改变氧化剂的大小，

更新日期：2018-03-07

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