Preparation of ordered mesoporous carbon material based on poly-dopamine and its application in selective enrichment of N-linked glycans†,Analytical Methods

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Preparation of ordered mesoporous carbon material based on poly-dopamine and its application in selective enrichment of N-linked glycans†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1039/c7ay02800h
Yameng Zhao _{1,

2,

3,

4,

5} , Yangyang Gan _{1,

2,

3,

4,

5} , Lingyi Zhang _{1,

2,

3,

4,

5} , Zhanying Chu _{1,

2,

3,

4,

5} , Fan Liu _{5,

6,

7,

8} , Weibing Zhang _{1,

2,

3,

4,

5}

Affiliation

Protein glycosylation has been demonstrated to be associated with numerous diseases. Taking into consideration of low abundance of N-linked glycans, the pretreatment of a biological sample is a key problem. In this study, a highly ordered mesoporous carbon material was prepared using SBA-15 as a hard template and poly-dopamine as a carbon source. The material showed ordered mesoporous structure, high specific area (1223 m² g⁻¹) with a pore size of 2.67 nm, high selectivity for glycans from glycoprotein, and good chemical inertness and mechanical strength. Based on the specific interaction between the carbon material and glycans and the size-exclusive mechanism, 25 N-linked glycans from OVA digest were efficiently captured. In addition, 30 N-linked glycans were selectively captured from human serum, which indicated that the prepared material is a promising tool in protein glycosylation research.

中文翻译：

基于聚多巴胺的有序介孔碳材料的制备及其在N-连接聚糖选择性富集中的应用†

蛋白质糖基化已被证明与多种疾病有关。考虑到N-连接的聚糖的低丰度，生物样品的预处理是关键问题。在这项研究中，使用SBA-15作为硬模板和聚多巴胺作为碳源，制备了高度有序的介孔碳材料。该材料显示出有序的介孔结构，高比表面积（1223 m ² g ^-1），孔径为2.67 nm，对糖蛋白中的聚糖具有高选择性以及良好的化学惰性和机械强度。基于碳材料和聚糖之间的特定相互作用以及尺寸排他的机理，25 N从OVA消化中获得的连接聚糖被有效地捕获。此外，从人血清中选择性地捕获了30个N-连接的聚糖，这表明所制备的材料是蛋白质糖基化研究中的有前途的工具。

更新日期：2018-01-17

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