Detection of the Cytotoxic Penitrems A–F in Cheese from the European Single Market by HPLC-MS/MS,Journal of Agricultural and Food Chemistry

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Detection of the Cytotoxic Penitrems A–F in Cheese from the European Single Market by HPLC-MS/MS
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-25 00:00:00 , DOI: 10.1021/acs.jafc.7b06001
Svetlana A. Kalinina _{1,

2} , Annika Jagels ₁ , Sebastian Hickert _{1,

2} , Lucas M. Mauriz Marques ₃ , Benedikt Cramer ₁ , Hans-Ulrich Humpf _{1,

2}

Affiliation

Penitrems are fungal indole diterpene-derived tremorgenic secondary metabolites, which are mainly produced by Penicillium spp. Several cases of intoxications with penitrems and subsequent occurrences of penitrem A in foodstuff underline the need for reliable quantitation methods for the detection of these mycotoxins in food. In this study, a simple and fast high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method for the quantitative analysis of penitrems A–F in cheese was developed. Therefore, penitrems A–F were isolated from Penicillium crustosum as analytical reference standards. The analysis of 60 cheese samples from the European single market (EU) revealed the occurrence of penitrem A in 10% of the analyzed samples with an average concentration of 28.4 μg/kg and a maximum concentration of 429 μg/kg. In addition to penitrem A, other members of the group of penitrems, namely, penitrems B, C, D, E, and F, were for the first time quantitatively detected in food samples, although in lower concentrations and with lower incidence in comparison to penitrem A. Moreover, we report cytotoxic effects of all penitrems on two cell lines (HepG2 and CCF-STTG1). This clearly underlines their relevance and the importance to analyze food samples in order to get insights into the human exposure toward these mycotoxins.

中文翻译：

HPLC-MS / MS法检测欧洲单一市场奶酪中的细胞毒性Penitrems A–F

青霉菌是真菌吲哚二萜衍生的震颤次生代谢产物，主要由青霉属产生。在食品中出现了数以百日咳杆菌中毒以及随后出现的百日咳杆菌A的案例，这表明需要可靠的定量方法来检测食品中的这些霉菌毒素。在这项研究中，开发了一种简单快速的高效液相色谱-串联质谱法（HPLC-MS / MS），用于定量分析奶酪中的Penitrems AF。因此，从青霉菌中分离出青霉菌AF作为分析参考标准。对来自欧洲单一市场（EU）的60个奶酪样品的分析显示，在10％的分析样品中出现了Penitrem A，平均浓度为28.4μg/ kg，最大浓度为429μg/ kg。除了青霉菌素A以外，首次在食品样品中定量检测到了青霉菌群中的其他成员，即青霉菌素B，C，D，E和F，尽管与之相比浓度较低且发生率较低。 PenitremA。此外，我们报告了所有Penitrem对两种细胞系（HepG2和CCF-STTG1）的细胞毒性作用。这清楚地强调了它们的相关性和分析食品样本的重要性，以便深入了解人类对这些真菌毒素的接触。

更新日期：2018-01-25

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