Abstract The investigation first time reports the efficacy of de-oiled algal biomass extract (DOABE) for mixotrophic cultivation of Chlorella sp. MCC27 to enhance biodiesel production in open tray systems and in BioXpert-V2 software connected photobioreactor (PBR). The cultivation systems with addition of DOABE (PBR systems + DOABE and open tray system + DOABE) as media were tested for quality and quantity of biodiesel. The presence of organic carbon and low nitrogen in DOABE caused >2 folds higher biomass productivity and >4 folds enhanced lipid productivity for the cells cultivated in PBR system + DOABE and open tray system + DOABE as compared to control (i.e. BBM). Biochemical analysis of cells from both the systems revealed the decrease in total carbohydrates and protein contents. The FAMEs analyses showed vehicular quality biodiesel. PBR system + DOABE showed edge over open tray system + DOABE in terms of biomass productivity and lipid content. The physical properties of biodiesel produced from Chlorella sp. MCC27 were more close to the fuel standards (ASTM D6751), when cells were cultivated in open tray system + DOABE than in PBR system + DOABE. Collectively, this study highlights the use of DOABE as a low cost feedstock for enhancing vehicular quality biodiesel production from microalgae.

中文翻译：

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利用脱油藻类生物质提高小球藻生产车辆质量生物柴油。在混合营养培养系统中

摘要 该研究首次报道了脱油藻类生物质提取物 (DOABE) 对小球藻混合营养培养的功效。MCC27 用于提高开放式托盘系统和 BioXpert-V2 软件连接光生物反应器 (PBR) 中生物柴油的生产。添加 DOABE 的培养系统（PBR 系统 + DOABE 和开放式托盘系统 + DOABE）作为培养基进行了生物柴油的质量和数量测试。与对照（即 BBM）相比，在 DOABE 中有机碳和低氮的存在导致在 PBR 系统 + DOABE 和开放式托盘系统 + DOABE 中培养的细胞的生物量生产力高出 2 倍以上，脂质生产力提高了 4 倍。来自这两个系统的细胞的生化分析显示总碳水化合物和蛋白质含量减少。FAMEs 分析显示了车辆质量的生物柴油。PBR 系统 + DOABE 在生物质生产力和脂质含量方面优于开放式托盘系统 + DOABE。由小球藻生产的生物柴油的物理特性。当细胞在开放式托盘系统 + DOABE 中培养而不是在 PBR 系统 + DOABE 中培养时，MCC27 更接近燃料标准 (ASTM D6751)。总的来说，这项研究强调了使用 DOABE 作为一种低成本原料来提高微藻生产车辆质量生物柴油。