AbstractThe authors describe a fluorescence polarization assay for HIV-DNA. It is based on the use of gold nanoparticles (AuNPs) modified with DNA dendritic macromolecules that act as signal amplifiers. In the presence of HIV-DNA, the AuNP-DNA dendritic macromolecules and fluorescently labeled DNA probe combine with HIV-DNA in a sandwich format to form a conjugate. This reaction slows down the rotational speed of the labeled DNA probe because of the increase of molecular weight and volume. This increases fluorescence polarization and the sensitivity of the system. The relative fluorescence polarization values increase linearly in the 150 pM to 6 nM HIV-DNA concentration range, with a 73 pM detection limit. The results show this amplification strategy to be most useful for ultrasensitive determination of oligonucleotides by means of fluorescence polarization. Graphical abstractSchematic of a novel fluorescence polarization assay for the HIV-DNA. Ultrasensitive detection is accomplished by using AuNP-DNA dendritic macromolecules as signal amplification factor.

中文翻译：

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基于使用树突修饰的金纳米粒子作为信号放大器的 HIV-DNA 荧光偏振基因检测

摘要作者描述了一种用于 HIV-DNA 的荧光偏振测定。它基于使用作为信号放大器的 DNA 树突状大分子修饰的金纳米粒子 (AuNP)。在 HIV-DNA 存在的情况下，AuNP-DNA 树突状大分子和荧光标记的 DNA 探针以夹心形式与 HIV-DNA 结合形成共轭物。由于分子量和体积的增加，该反应减慢了标记 DNA 探针的旋转速度。这增加了荧光偏振和系统的灵敏度。相对荧光偏振值在 150 pM 至 6 nM HIV-DNA 浓度范围内线性增加，检测限为 73 pM。结果表明，这种扩增策略对于通过荧光偏振法对寡核苷酸的超灵敏测定最为有用。图形摘要用于 HIV-DNA 的新型荧光偏振测定的示意图。超灵敏检测是通过使用 AuNP-DNA 树突状大分子作为信号放大因子来实现的。