Statistical Approach for the Identification of Cellulolytic Enzyme Inhibitors Using Switchgrass Dilute Acid Prehydrolyzates as a Model System,ACS Sustainable Chemistry & Engineering

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Statistical Approach for the Identification of Cellulolytic Enzyme Inhibitors Using Switchgrass Dilute Acid Prehydrolyzates as a Model System
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acssuschemeng.7b03686
Angele Djioleu ₁ , Danielle Julie Carrier ₂

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The identification of biomass pretreatment-generated compounds that impede cellulose hydrolysis is critical for improving the overall biomass saccharification process. The aim of this study was to correlate the identification and concentrations of switchgrass dilute acid pretreatment-generated compounds to cellulolytic enzyme inhibition and to tie this back to processing parameters. Preparation of 24 dilute acid prehydrolyzates was performed with switchgrass at temperatures from 140 to 180 °C, processing times from 10 to 40 min, and sulfuric acid concentrations of 0.5% or 1% (v/v). Results showed that all the switchgrass prehydrolyzates significantly reduced cellulolytic enzyme activities when assayed against model substrates. Exoglucanase was the most sensitive with its activity reduction, ranging from 58% to 88%; the inhibitory effect on β-glucosidase and the cellulase cocktail ranged from 32% to 63% and 16% to 41%, respectively. Polyphenolic compounds were the most detrimental pretreatment-generated products to the cellulolytic enzymes, especially to exoglucanase. Limited enzyme inhibition, with acceptable biomass digestibility, was observed with pretreatment conditions corresponding to 160 °C. The statistically based approach used in this study proved to be a valid method to assess the effect of pretreatment-generated compounds on cellulolytic enzyme activities, linking their generation to pretreatment processing parameters.

中文翻译：

使用柳枝D稀酸预水解物作为模型系统鉴定纤维素分解酶抑制剂的统计方法

阻碍纤维素水解的生物质预处理生成的化合物的鉴定对于改善整体生物质糖化过程至关重要。这项研究的目的是将柳枝di稀酸预处理生成的化合物的鉴定和浓度与纤维素分解酶抑制作用相关联，并将其与加工参数联系起来。用柳枝switch在140至180°C的温度，10至40分钟的处理时间以及0.5％或1％（v / v）的硫酸浓度下制备24种稀酸预水解物。结果表明，当对模型底物进行测定时，所有柳枝pre的预水解产物均显着降低了纤维素分解酶的活性。葡聚糖酶最敏感，其活性降低范围从58％到88％。对β-葡萄糖苷酶和纤维素酶混合物的抑制作用分别为32％至63％和16％至41％。多酚化合物是纤维素分解酶（尤其是外切葡聚糖酶）中最有害的预处理生成产物。在相当于160°C的预处理条件下，观察到了有限的酶抑制作用和可接受的生物质消化率。在本研究中使用的基于统计的方法被证明是评估预处理生成的化合物对纤维素分解酶活性的影响的有效方法，并将其生成与预处理工艺参数联系起来。在相当于160°C的预处理条件下，观察到了有限的酶抑制作用和可接受的生物质消化率。在本研究中使用的基于统计的方法被证明是评估预处理生成的化合物对纤维素分解酶活性的影响的有效方法，并将其生成与预处理工艺参数联系起来。在相当于160°C的预处理条件下，观察到了有限的酶抑制作用和可接受的生物质消化率。在本研究中使用的基于统计的方法被证明是评估预处理生成的化合物对纤维素分解酶活性的影响的有效方法，并将其生成与预处理工艺参数联系起来。

更新日期：2018-01-16

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