Analysis of coffee seed vigor by extractive electrospray ionization mass spectrometry,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Analysis of coffee seed vigor by extractive electrospray ionization mass spectrometry
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-15 00:00:00 , DOI: 10.1039/c7ay02403g
Yalian Zhou _{1,

2,

3,

4} , Meng Cui _{1,

2,

3,

4} , Qin Yin _{1,

2,

3,

4} , Min Deng _{1,

2,

3,

4} , Yingbin Hao _{1,

2,

3,

4} , Xueyong Huang _{1,

2,

3,

4} , Liping Luo _{1,

2,

3,

4,

5}

Affiliation

Water extracts of coffee seeds of various ages were analyzed in positive ion mode by extractive electrospray ionization mass spectrometry (EESI-MS) without any sample pretreatment, and the typical metabolites were identified by tandem mass spectrometry. Further assessment using principal component analysis (PCA), cluster analysis (CA), and discriminant analysis (DA) demonstrated that a combination of EESI-MS and multivariate analysis could effectively distinguish coffee seeds with different vigor. In PCA, the first three principal components explained 99.3% of the total variation, and coffee seeds with the same vigor were clustered closely together in CA with an accuracy of 100%. In DA, both the accuracy rate of discrimination of the training samples and the success rate of cross validation analysis were 100%. We concluded that coffee seeds with diverse vigor could be differentiated by EESI-MS coupled with multivariate analysis, providing a novel, sensitive, and rapid measurement of seed vigor in coffee.

中文翻译：

萃取电喷雾电离质谱法分析咖啡种子活力

在没有任何样品预处理的情况下，通过萃取电喷雾电离质谱法（EESI-MS）以正离子模式分析了不同年龄的咖啡种子的水提取物，并通过串联质谱法鉴定了典型的代谢物。使用主成分分析（PCA），聚类分析（CA）和判别分析（DA）进行的进一步评估表明，结合使用EESI-MS和多变量分析可以有效地区分不同活力的咖啡种子。在PCA中，前三个主要成分解释了总变异的99.3％，并且具有相同活力的咖啡种子在CA中紧密聚集在一起，准确度为100％。在DA中，训练样本的判别准确率和交叉验证分析的成功率均为100％。

更新日期：2018-01-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11