Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.

中文翻译：

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在一系列纳米孔上高度平行的直接RNA测序

对生物样品中的RNA进行测序可以解锁大量信息，包括细菌和病毒的身份，替代剪接的细微差别或生物的转录状态。然而，由于短的读取长度和逆转录或扩增偏差，当前的方法具有局限性。在这里，我们展示了纳米孔直接RNA序列，这是一种高度并行，实时，单分子的方法，可绕过逆转录或扩增步骤。该方法可产生全长，链特异性的RNA序列，并能够直接检测RNA中的核苷酸类似物。