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Cyclodextrin-modified nanodiamond for the sensitive fluorometric determination of doxorubicin in urine based on its differential affinity towards β/γ-cyclodextrins
Microchimica Acta ( IF 5.3 ) Pub Date : 2018-01-15 , DOI: 10.1007/s00604-017-2660-y
M. Laura Soriano , Carolina Carrillo-Carrion , Celia Ruiz-Palomero , Miguel Valcárcel

AbstractThe manuscript reports on the preparation of β-cyclodextrin-modified nanodiamonds (βCD-ND) for the extraction and preconcentration of the fluorescent anticancer drug doxorubicin (DOX) from biological samples. The inclusion of DOX into the cavities of β- and γ-cyclodextrin (CD) confirms their utility for selective extraction and elution of the drug based on its good fit to the cyclodextrin cavity. Although both larger cyclodextrins (βCD and γCD) accommodate DOX, DOX clearly prefers the bigger γCD cavities. Dispersive micro solid-phase extraction using βCD-ND as sorbent enables the inclusion complexation of DOX. The elution of DOX from βCD-ND cavities occurs with a basic solution of γCD containing 10% acetonitrile owing to the preferential affinity (i.e. optimal fit) of DOX into the larger γCD cavity. DOX is quantified by monitoring its intrinsic fluorescence (exc/em = 475/595 nm). The method can determine DOX in urine with a limit of detection of 18 ng·mL−1. Recoveries (93.2% and 94.0%) and precision (RSDs of 5.9% and 4.7%) at 100 and 400 ng·mL−1 DOX levels in urine are satisfactory. The matrix effect is negligible even when working with undiluted urine samples. Graphical abstractNanodiamonds functionalized with β-cyclodextrin (βCD-ND) were used as sorbent for the determination of nanomolar levels of doxorubicin (DOX). It is based on host:guest interactions ruled by different stabilities of DOX within cyclodextrin (CD) cavity-size: βCD/γCD.

中文翻译:

环糊精修饰的纳米金刚石基于其对 β/γ-环糊精的不同亲和力,用于尿液中阿霉素的灵敏荧光测定

摘要该手稿报告了 β-环糊精修饰的纳米金刚石 (βCD-ND) 的制备,用于从生物样品中提取和预浓缩荧光抗癌药物阿霉素 (DOX)。将 DOX 包含在 β- 和 γ-环糊精 (CD) 的空腔中,证实了它们可用于选择性提取和洗脱药物,因为它与环糊精空腔良好契合。尽管较大的环糊精(βCD 和 γCD)都能容纳 DOX,但 DOX 显然更喜欢较大的 γCD 腔。使用 βCD-ND 作为吸附剂的分散微固相萃取能够实现 DOX 的包合络合。由于 DOX 与较大的 γCD 腔的优先亲和力(即最佳配合),DOX 从 βCD-ND 腔中的洗脱发生在含有 10% 乙腈的 γCD 碱性溶液中。DOX 通过监测其固有荧光 (exc/em = 475/595 nm) 进行量化。该方法可测定尿液中的 DOX,检测限为 18 ng·mL-1。尿液中 100 和 400 ng·mL-1 DOX 水平的回收率(93.2% 和 94.0%)和精密度(RSD 为 5.9% 和 4.7%)令人满意。即使使用未稀释的尿液样本,基质效应也可以忽略不计。图形摘要 用 β-环糊精 (βCD-ND) 功能化的纳米金刚石用作吸附剂,用于测定纳摩尔水平的阿霉素 (DOX)。它基于由环糊精 (CD) 腔大小:βCD/γCD 内 DOX 的不同稳定性决定的宿主:客体相互作用。7%) 在 100 和 400 ng·mL-1 DOX 水平的尿液中是令人满意的。即使使用未稀释的尿液样本,基质效应也可以忽略不计。图形摘要 用 β-环糊精 (βCD-ND) 功能化的纳米金刚石用作吸附剂,用于测定纳摩尔水平的阿霉素 (DOX)。它基于由环糊精 (CD) 腔大小:βCD/γCD 内 DOX 的不同稳定性决定的宿主:客体相互作用。7%) 在 100 和 400 ng·mL-1 DOX 水平的尿液中是令人满意的。即使使用未稀释的尿液样本,基质效应也可以忽略不计。图形摘要 用 β-环糊精 (βCD-ND) 功能化的纳米金刚石用作吸附剂,用于测定纳摩尔水平的阿霉素 (DOX)。它基于由环糊精 (CD) 腔大小:βCD/γCD 内 DOX 的不同稳定性决定的宿主:客体相互作用。
更新日期:2018-01-15
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