Total reflection X-ray fluorescence based quantification of gold nanoparticles in cancer cells,Journal of Analytical Atomic Spectrometry

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Total reflection X-ray fluorescence based quantification of gold nanoparticles in cancer cells
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2018-01-15 00:00:00 , DOI: 10.1039/c7ja00332c
Gabriella Mankovskii _{1,

2,

3,

4,

5} , Ana Pejović-Milić _{1,

2,

3,

4,

5}

Affiliation

In the last two decades, nanoparticles of different shapes, sizes and materials have been investigated for various nanomedicine applications, ranging from imaging to radiation therapy, in efforts to improve conventional cancer therapies. The current focus of nanomedicine is to increase the delivery and cellular uptake of nanoparticles. Thus, further development and advancement of the field requires accurate means to quantitatively assess nanoparticle concentration in cells. Inductively Coupled Plasma (ICP) based methods are currently being used for nanoparticle quantification. Such methods however require extensive sample preparation and large sample volumes, which poses a challenge when dealing with small sample volumes with low concentration. This work describes the development and validation of a total reflection X-ray fluorescence (TXRF) based quantification method for trace-level gold nanoparticles in an organic matrix. Suitable internal standards, fitting approaches and sample preparation methods that yield acceptable recovery rates were investigated. The developed method was validated with reference material nanoparticles. Recovery rates of (102.7 ± 3.7)% and (100.9 ± 5.1)% were achieved for nanoparticles in an ionic solution and organic matrix respectively. These results suggest that TXRF is an adequate technique to accurately quantify gold nanoparticle uptake in cancer cells.

中文翻译：

基于全反射X射线荧光的癌细胞中金纳米颗粒的定量

在过去的二十年中，已经对各种形状，大小和材料的纳米颗粒进行了研究，以用于从成像到放射治疗的各种纳米医学应用，以努力改善常规的癌症治疗方法。纳米医学的当前焦点是增加纳米颗粒的递送和细胞摄取。因此，该领域的进一步发展和进步需要精确的方法来定量评估细胞中的纳米颗粒浓度。基于电感耦合等离子体（ICP）的方法目前正用于纳米颗粒定量。然而，这样的方法需要大量的样品制备和大的样品量，这在处理小样品量和低浓度时提出了挑战。这项工作描述了基于全反射X射线荧光（TXRF）的有机基质中痕量金纳米颗粒定量方法的开发和验证。研究了产生可接受的回收率的合适的内标，拟合方法和样品制备方法。所开发的方法已通过参考材料纳米粒子验证。纳米粒子在离子溶液和有机基质中的回收率分别达到（102.7±3.7）％和（100.9±5.1）％。这些结果表明，TXRF是准确定量癌细胞中金纳米颗粒摄取的适当技术。研究了产生可接受回收率的拟合方法和样品制备方法。所开发的方法已通过参考材料纳米粒子验证。纳米粒子在离子溶液和有机基质中的回收率分别达到（102.7±3.7）％和（100.9±5.1）％。这些结果表明，TXRF是准确定量癌细胞中金纳米颗粒摄取的适当技术。研究了产生可接受回收率的拟合方法和样品制备方法。所开发的方法已通过参考材料纳米粒子验证。纳米粒子在离子溶液和有机基质中的回收率分别达到（102.7±3.7）％和（100.9±5.1）％。这些结果表明，TXRF是准确定量癌细胞中金纳米颗粒摄取的适当技术。

更新日期：2018-01-15

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