Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells,Small

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Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells
Small ( IF 13.0 ) Pub Date : 2018-01-15 , DOI: 10.1002/smll.201702955
Liyuan Zhang _{1,

2} , Kaiwen Chen ₁ , Haoyue Zhang ₁ , Bo Pang _{1,

3} , Chang-Hyung Choi ₂ , Angelo S. Mao _{2,

4} , Hongbing Liao ₃ , Stefanie Utech ₂ , David J. Mooney _{2,

4} , Huanan Wang ₁ , David A. Weitz _{2,

4,

5}

Affiliation

Controlled encapsulation and pairing of single cells within a confined 3D matrix can enable the replication of the highly ordered cellular structure of human tissues. Microgels with independently controlled compartments that can encapsulate cells within separately confined hydrogel matrices would provide precise control over the route of pairing single cells. Here, a one‐step microfluidic method is presented to generate monodisperse multicompartment microgels that can be used as a 3D matrix to pair single cells in a highly biocompatible manner. A method is presented to induce microgels formation on chip, followed by direct extraction of the microgels from oil phase, thereby avoiding prolonged exposure of the microgels to the oil. It is further demonstrated that by entrapping stem cells with niche cells within separate but adjacent compartments of the microgels, it can create complex stem cell niche microenvironments in a controlled manner, which can serve as a useful tool for the study of cell–cell interactions. This microfluidic technique represents a significant step toward high‐throughput single cells encapsulation and pairing for the study of intercellular communications at single cell level, which is of significant importance for cell biology, stem cell therapy, and tissue engineering.

中文翻译：

用于3D封装和单细胞配对的微流控模板多室微凝胶

在受限的3D矩阵内，单个细胞的受控封装和配对可以实现人体组织高度有序的细胞结构的复制。具有可以将细胞封装在单独的密闭水凝胶基质中的独立可控隔室的微凝胶将提供对配对单个细胞的途径的精确控制。在这里，提出了一种单步微流体方法来生成单分散多室微凝胶，该凝胶可用作3D基质，以高度生物相容的方式配对单个细胞。提出了一种诱导微凝胶在芯片上形成，然后从油相中直接提取微凝胶的方法，从而避免了微凝胶长时间暴露于油中。进一步证明，通过在微凝胶的单独但相邻的区室中将干细胞与小生境细胞截留在一起，它可以以可控的方式创建复杂的干细胞小生境微环境，这可以用作研究细胞间相互作用的有用工具。这种微流体技术代表了高通量单细胞封装和配对研究的重要一步，该研究对于单细胞水平的细胞间通讯研究而言，对细胞生物学，干细胞治疗和组织工程具有重要意义。

更新日期：2018-01-15

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