Proteomic Profiling of Leishmania donovani Promastigote Subcellular Organelles,Journal of Proteome Research

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Proteomic Profiling of Leishmania donovani Promastigote Subcellular Organelles
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00817
Armando Jardim ₁ , Darryl B. Hardie ₂ , Jan Boitz ₃ , Christoph H. Borchers _{2,

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Affiliation

To facilitate a greater understanding of the biological processes in the medically important Leishmania donovani parasite, a combination of differential and density-gradient ultracentrifugation techniques were used to achieve a comprehensive subcellular fractionation of the promastigote stage. An in-depth label-free proteomic LC–MS/MS analysis of the density gradients resulted in the identification of ∼50% of the Leishmania proteome (3883 proteins detected), which included ∼645 integral membrane proteins and 1737 uncharacterized proteins. Clustering and subcellular localization of proteins was based on a subset of training Leishmania proteins with known subcellular localizations that had been determined using biochemical, confocal microscopy, or immunoelectron microscopy approaches. This subcellular map will be a valuable resource that will help dissect the cell biology and metabolic processes associated with specific organelles of Leishmania and related kinetoplastids.

中文翻译：

利什曼原虫前鞭毛体亚细胞器的蛋白质组学分析

为了促进对医学上重要的利什曼原虫多虫的寄生虫的生物学过程的更多了解，使用了差分和密度梯度超速离心技术的组合来实现前鞭毛体阶段的全面亚细胞分级分离。深入的无标记蛋白质组学LC-MS / MS分析密度梯度，可鉴定出约50％的利什曼原虫蛋白质组（检测到3883种蛋白质），其中包括645种完整的膜蛋白和1737种未鉴定的蛋白。蛋白质的聚类和亚细胞定位是基于训练利什曼原虫的一个子集具有已知亚细胞定位的蛋白质，已使用生化，共聚焦显微镜或免疫电子显微镜方法确定。该亚细胞图将是宝贵的资源，将有助于剖析与利什曼原虫的特定细胞器和相关运动型胶体相关的细胞生物学和代谢过程。

更新日期：2018-02-06

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