The enzymatic synthesis of fine chemicals is a promising approach to overcome the major problems of the traditional chemical routes and implement eco‐friendly and low energy consuming industrial processes. This work introduces for the first time the one‐step production of an analogue of the sperm whale's spermaceti in a vacuum, solvent‐free system. With the aim of easing the industrial feasibility of the process, two commercial biocatalysts with appropriate technical properties were tested. Results pointed out that under optimal conditions, both commercial immobilized derivatives (Lipozyme^® RM IM and CalB immo Plus) gave excellent results as they can be reused up to 15 batches without a noticeable loss of their activity, obtaining conversions of ∼98% in two hours. This green and sustainable process might be economically competitive and leads to a product that meets the all specifications required for its cosmetic use and that can be labeled as “natural”, increasing its added value.

中文翻译：

---

使用商业化固定化脂肪酶的无溶剂精子一步生产

精细化学品的酶促合成是克服传统化学路线的主要问题并实施生态友好且能耗低的工业过程的一种有前途的方法。这项工作首次引入了在真空，无溶剂的系统中一步一步生产抹香鲸的乙酰丙酮类似物的方法。为了减轻该方法的工业可行性，测试了两种具有适当技术性能的商业生物催化剂。结果指出，在最佳条件下，无论是商业固定化衍生物（的Lipozyme ^® RM IM和CalB immo Plus）提供了出色的结果，因为它们可以重复使用多达15批，而活性没有明显损失，在两个小时内获得了约98％的转化率。这种绿色且可持续的过程可能在经济上具有竞争力，并导致产品达到其化妆品用途所需的所有规格，并且可以贴上“天然”标签，从而增加了附加值。