Complex spatial working memory tasks have been shown to require both hippocampal sharp-wave ripple (SWR) activity and dentate gyrus (DG) neuronal activity. We therefore asked whether DG inputs to CA3 contribute to spatial working memory by promoting SWR generation. Recordings from DG and CA3 while rats performed a dentate-dependent working memory task on an eight-arm radial maze revealed that the activity of dentate neurons and the incidence rate of SWRs both increased during reward consumption. We then found reduced reward-related CA3 SWR generation without direct input from dentate granule neurons. Furthermore, CA3 cells with place fields in not-yet-visited arms preferentially fired during SWRs at reward locations, and these prospective CA3 firing patterns were more pronounced for correct trials and were dentate-dependent. These results indicate that coordination of CA3 neuronal activity patterns by DG is necessary for the generation of neuronal firing patterns that support goal-directed behavior and memory.

中文翻译：

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齿状网络活动通过支持 CA3 尖波波纹生成和 CA3 神经元的前瞻性放电，对于空间工作记忆是必要的。


复杂的空间工作记忆任务已被证明需要海马尖波波纹（SWR）活动和齿状回（DG）神经元活动。因此，我们询问 DG 对 CA3 的输入是否通过促进 SWR 的生成来促进空间工作记忆。当大鼠在八臂径向迷宫上执行齿状依赖工作记忆任务时，DG 和 CA3 的记录显示，在奖励消耗过程中，齿状神经元的活动和 SWR 的发生率均增加。然后我们发现，在没有齿状颗粒神经元直接输入的情况下，奖励相关的 CA3 SWR 生成减少。此外，在 SWR 期间，尚未访问的手臂中具有位置场的 CA3 细胞会优先在奖励位置的 SWR 中放电，并且这些预期的 CA3 放电模式对于正确的试验更为明显，并且是齿状依赖性的。这些结果表明，DG 协调 CA3 神经元活动模式对于生成支持目标导向行为和记忆的神经元放电模式是必要的。