Streptococcus pneumoniae causes life‐threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are reported. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi‐ and fully‐synthetic glycoconjugate vaccines.

中文翻译：

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使用合成寡糖鉴定肺炎链球菌7F荚膜多糖的最小糖基

肺炎链球菌引起威胁生命的疾病，包括脑膜炎，肺炎和败血症。基于纯化荚膜多糖的现有糖缀合物疫苗被广泛使用，每年可帮助预防数百万人的死亡。在此，报道了类似于肺炎链球菌血清型7F（ST7F）荚膜多糖重复单元的部分的寡糖的总合成。为了定义最小的聚糖表位，含有合成寡糖的聚糖微阵列用于筛选人参血清，并揭示了ST7F的两个侧链在抗原识别中起关键作用。保护性最小表位的鉴定对于设计有效的半合成和全合成糖缀合物疫苗至关重要。