Recently, biological materials have attracted more and more attentions on preparing the surface-enhanced Raman spectroscopy (SERS) substrate with the conspicuous SERS performance. Herein, we demonstrate a simply facile and inexpensive approach to fabricate a novel natural SERS substrate based on graphene oxide-Ag nanoparticles-Mytilus coruscus (GO-AgNPs-M.c.). The surface-enhanced resonance Raman spectroscopy (SERRS) signals of the Rhodamine 6G (R6G) on the GO-AgNPs-M.c. hybrids exhibit the much better SERRS performances in terms of the sensitivity, the signal reproducibility and the antioxidant stability than that on the AgNPs-M.c. one. The well electric field enhancement contribution is likewise confirmed in theory via the COMSOL software. Moreover, the successful detection of trace malachite green (MG) and methylene blue (MB) indicates the well feasibility for multi-molecules detection on the GO-AgNPs-M.c. hybrids. Thus, this inexpensive and high-efficiency natural SERS substrate have great potential for the considerable biochemical SERS applications and broaden the way for preparing multiple SERS platforms derived from other natural materials.

近年来，生物材料在制备具有显着SERS性能的表面增强拉曼光谱（SERS）基底方面引起了越来越多的关注。在这里，我们展示了一种简单易行且便宜的方法来制造基于氧化石墨烯-Ag纳米颗粒-Mytilus coruscus（GO-AgNPs-Mc）的新型天然SERS基质。罗丹明6G（R6G）在GO-AgNPs-Mc杂交体上的表面增强共振拉曼光谱（SERRS）信号在灵敏度，信号重现性和抗氧化剂稳定性方面比在AgNPs上表现出更好的SERRS性能-麦克一。理论上也通过COMSOL软件确认了井电场增强作用。而且，成功检测出痕量的孔雀石绿（MG）和亚甲基蓝（MB）表明在GO-AgNPs-Mc杂种上进行多分子检测具有很好的可行性。因此，这种廉价且高效的天然SERS底物在大量生化SERS应用中具有巨大潜力，并拓宽了制备多种衍生自其他天然材料的SERS平台的途径。