In this article, some new peculiarities and prospects of cysteine determination using aggregation of label-free gold nanoparticles (AuNPs) are described. Features of the aggregation both in solution and on polyurethane surface are compared. Effects of ethylenediaminetetraacetate (EDTA) and cysteamine on the aggregation of AuNPs, which increase sensitivity, are considered. In case of EDTA, it was simultaneously possible to eliminate interferences from cations of metals. It is shown that attaching AuNPs onto polyurethane foam surface changes their sensitivity and selectivity, which can be used for the determination of cysteine. Based on found peculiarities, new methods for the determination of cysteine in a range 0.06–0.5 μg mL⁻¹ (LOD = 0.04 μg mL⁻¹) by spectrophotometry, and 0.02–0.15 μg mL⁻¹ (LOD = 0.01 μg mL⁻¹) by diffuse reflectance spectroscopy were developed.

在本文中，描述了使用无标记金纳米颗粒（AuNPs）聚集进行半胱氨酸测定的一些新特性和前景。比较了溶液中和聚氨酯表面上的聚集特征。考虑了乙二胺四乙酸盐（EDTA）和半胱胺对AuNP聚集的影响，这会增加灵敏度。如果使用EDTA，则可以同时消除金属阳离子的干扰。结果表明，将AuNPs附着在聚氨酯泡沫表面会改变其灵敏度和选择性，可用于测定半胱氨酸。基于找到的特殊性，将半胱氨酸在一个范围0.06-0.5微克毫升的判定新方法^-1（LOD = 0.04微克毫升^-1用分光光度法测定，并用漫反射光谱法测定0.02–0.15μgmL ^-1（LOD = 0.01μgmL ^-1）。