An accurate, rapid, and sensitive method for the determination of risperidone and 9-hydroxyrisperidone in urine samples was developed by combining water-compatible magnetic molecularly imprinted solid-phase extraction with high performance liquid chromatography. Several variables relating to the efficiency of magnetic solid phase extraction were optimized, including the amount of adsorbent, adsorption time, type of elution solvent, and desorption time. The analytical performance of this method was validated under the optimized conditions. The linearity for risperidone and 9-hydroxyrisperidone was obtained in the range 1–2000 ng mL⁻¹ with correlation coefficient ≥ 0.991. Limits of detection of risperidone and 9-hydroxyrisperidone are 0.21 ng mL⁻¹ and 0.24 ng mL⁻¹, respectively. Recoveries at three spike levels (10, 100, and 1000 ng mL⁻¹) ranged from 94.6% to 102.4% with relative standard deviations (%) ≤ 5.3. These results confirmed that this method can be successfully and facilely used to analyze the multi-residues of risperidone and 9-hydroxyrisperidone in urine samples with high efficiency and good sensitivity.

结合水相容性磁性分子印迹固相萃取与高效液相色谱法，开发了一种准确，快速，灵敏的尿液中利培酮和9-羟基利培酮的测定方法。优化了与磁性固相萃取效率有关的几个变量，包括吸附剂的量，吸附时间，洗脱溶剂的类型和解吸时间。在优化条件下验证了该方法的分析性能。利培酮和9-羟基利培酮的线性范围为1-2000 ng mL ^-1，相关系数≥0.991。利培酮和9-羟基利培酮的检出限为0.21 ng mL ^-1和0.24 ng mL ^-1， 分别。三种加标水平（10、100和1000 ng mL ^-1）的回收率从94.6％到102.4％，相对标准偏差（％）≤5.3。这些结果证实了该方法可以成功，简便地用于尿液样品中利培酮和9-羟基利培酮的多残留分析，且效率高，灵敏度高。