The Cre–lox recombination approach is commonly used to generate cell-specific gene inactivation (or activation). We have noticed that the breeding and genotyping sections of papers utilizing Cre–lox techniques are frequently incomplete. While seemingly straightforward, there are important considerations that need to be implemented in the breeding and genotyping methods to prevent the introduction of experimental confounds. Germline recombination and transient expression of Cre recombinase during development are some examples of the complications that can occur, and conventional genotyping methods may fail to identify these events. In this opinion article, we highlight the importance of testing for unexpected recombination events, suggest strategies to isolate and minimize adverse recombination events, and encourage editors and reviewers to expect more definitive statements regarding the validation of genotyping.

Cre-lox 重组方法通常用于产生细胞特异性基因失活（或激活）。我们注意到，使用 Cre-lox 技术的论文的育种和基因分型部分经常是不完整的。虽然看似简单，但在育种和基因分型方法中需要实施一些重要的考虑因素，以防止引入实验混淆。Cre 重组酶在发育过程中的种系重组和瞬时表达是可能发生的并发症的一些例子，传统的基因分型方法可能无法识别这些事件。在这篇观点文章中，我们强调了测试意外重组事件的重要性，提出了隔离和最小化不良重组事件的策略，