Plants have the remarkable ability to drive cellular dedifferentiation and regeneration. Changes in epigenetic landscapes accompany the cell fate transition. Notably, modifications of chromatin structure occur primarily during callus formation via an in vitro tissue culture process and, thus, pluripotent callus cells have unique epigenetic signatures. Here, we highlight the latest progress in epigenetic regulation of callus formation in plants, which addresses fundamental questions related to cell fate changes and pluripotency establishment. Global and local modifications of chromatin structure underlie callus formation, and the combination and sequence of epigenetic modifications further shape intricate cell fate changes. This review illustrates how a series of chromatin marks change dynamically during callus formation and their biological relevance in plant regeneration.

植物具有驱动细胞去分化和再生的显着能力。表观遗传景观的变化伴随着细胞命运的转变。值得注意的是，染色质结构的修饰主要发生在通过体外形成愈伤组织的过程中组织培养过程以及因此多能愈伤组织细胞具有独特的表观遗传学特征。在这里，我们重点介绍植物愈伤组织形成的表观遗传调控的最新进展，该进展解决了与细胞命运变化和多能性建立有关的基本问题。染色质结构的整体和局部修饰是愈伤组织形成的基础，表观遗传修饰的组合和顺序进一步塑造了复杂的细胞命运变化。这篇综述说明了愈伤组织形成过程中一系列染色质标记是如何动态变化的，以及它们在植物再生中的生物学相关性。