Here, S237 was identified to be important in fine tuning the substrate specificity and enantioselectivity of alcohol dehydrogenase from Kluyveromyces polysporus (KpADH). In the reduction of a diaryl ketone, (4-chlorophenyl)-(pyridin-2-yl)-methanone (1a), the highest and lowest enantioselectivity of 96.1% and 27.0% e.e. (R) were obtained with S237A and S237C. Kinetic parameters analysis revealed that S237G, S237A, S237H and S237D displayed improved k_cat/K_m toward 1a. Various prochiral ketones, including acetophenone, 4-chloroacetophenone and ethyl 2-oxo-4-phenylbutyrate could be asymmetrically reduced by S237C, S237G and S237E with > 99% e.e. This study provides guidance for the application of KpADH in the preparation of chiral secondary alcohols.

在这里，S237被认为对微调多孢克鲁维酵母（Kp ADH）的醇脱氢酶的底物特异性和对映选择性很重要。在二芳基酮（4-氯苯基）-（吡啶-2-基）-甲酮（1a）的还原中，用S237A和S237C获得最高和最低对映选择性，分别为96.1％和27.0％ee（R）。动力学参数分析表明，S237G，S237A，S237H和S237D的k _cat / K _m值朝1a方向提高。。ee > 99％ee的S237C，S237G和S237E可以不对称还原包括乙酰苯，4-氯苯乙酮和2-氧代-4-苯基丁酸乙酯在内的各种手性酮。该研究为Kp ADH在手性仲代制备中的应用提供了指导酒精。