Photothermal‐Responsive Conjugated Polymer Nanoparticles for Remote Control of Gene Expression in Living Cells,Advanced Materials

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Photothermal‐Responsive Conjugated Polymer Nanoparticles for Remote Control of Gene Expression in Living Cells
Advanced Materials ( IF 27.4 ) Pub Date : 2018-01-12 , DOI: 10.1002/adma.201705418
Yunxia Wang _{1,

2} , Shengliang Li ₂ , Pengbo Zhang ₂ , Haotian Bai ₂ , Liheng Feng ₁ , Fengting Lv ₂ , Libing Liu ₂ , Shu Wang ₂

Affiliation

Remote control and noninvasive manipulation of cellular bioprocess has received intensive attention as a powerful technology to control cell functions. Here, a strategy is developed to remotely control intracellular gene expression with high spatial and temporal resolutions by using photothermal‐responsive conjugated polymer nanoparticles (CPNs) as the transducer under near‐infrared light irradiation. After being modified with positive charged peptide, the CPNs with superior photothermal conversion capacity could effectively coat on the surface of living cells and generate localized heat to trigger target gene expression. The heat‐inducible heat shock protein‐70 promoter starts transcription of downstream EGFP gene in response to heat shock, thus producing green fluorescent protein in the living cells. The combination of heat‐inducible gene promoter and photothermal‐responsive CPNs provides a method for the development of thermogenetics.

中文翻译：

光热响应共轭聚合物纳米粒子，用于远程控制活细胞中的基因表达

作为控制细胞功能的强大技术，细胞生物过程的远程控制和非侵入性操纵已受到广泛关注。在这里，通过使用光热响应共轭聚合物纳米颗粒（CPN）作为传感器在近红外光照射下，开发了一种以高时空分辨率远程控制细胞内基因表达的策略。带有正电荷的肽修饰后，具有优异光热转化能力的CPNs可以有效地覆盖在活细胞的表面，并产生局部热量来触发靶基因的表达。热诱导的热激蛋白70启动子响应热激而开始下游EGFP基因的转录，从而在活细胞中产生绿色荧光蛋白。

更新日期：2018-01-12

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