Metabolic Fingerprinting on a Plasmonic Gold Chip for Mass Spectrometry Based in Vitro Diagnostics,ACS Central Science

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Metabolic Fingerprinting on a Plasmonic Gold Chip for Mass Spectrometry Based in Vitro Diagnostics
ACS Central Science ( IF 12.7 ) Pub Date : 2018-01-12 00:00:00 , DOI: 10.1021/acscentsci.7b00546
Xuming Sun ₁ , Lin Huang ₁ , Ru Zhang ₁ , Wei Xu ₁ , Jingyi Huang ₁ , Deepanjali D Gurav ₁ , Vadanasundari Vedarethinam ₁ , Ruoping Chen ₁ , Jiatao Lou ₁ , Qian Wang ₁ , Jingjing Wan ₂ , Kun Qian ₁

Affiliation

Current metabolic analysis is far from ideal to engage clinics and needs rationally designed materials and device. Here we developed a novel plasmonic chip for clinical metabolic fingerprinting. We first constructed a series of chips with gold nanoshells on the surface through controlled particle synthesis, dip-coating, and gold sputtering for mass production. We integrated the optimized chip with microarrays for laboratory automation and micro-/nanoscaled experiments, which afforded direct high-performance metabolic fingerprinting by laser desorption/ionization mass spectrometry using 500 nL of various biofluids and exosomes. Further we for the first time demonstrated on-chip in vitro metabolic diagnosis of early stage lung cancer patients using serum and exosomes. This work initiates a new bionanotechnology based platform for advanced metabolic analysis toward large-scale diagnostic use.

中文翻译：

用于基于体外诊断的质谱分析的等离激元金芯片上的代谢指纹图谱

目前的代谢分析对于临床来说还远远不够理想，需要合理设计的材料和设备。在这里，我们开发了一种用于临床代谢指纹识别的新型等离子体芯片。我们首先通过受控粒子合成、浸涂、金溅射等方法构建了一系列表面带有金纳米壳的芯片，并进行了量产。我们将优化的芯片与用于实验室自动化和微/纳米级实验的微阵列集成，通过激光解吸/电离质谱法使用 500 nL 的各种生物流体和外泌体提供直接的高性能代谢指纹。此外，我们首次展示了使用血清和外泌体对早期肺癌患者进行芯片体外代谢诊断。这项工作开创了一个基于生物纳米技术的新平台，用于大规模诊断用途的高级代谢分析。

更新日期：2018-01-12

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