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One-Step Bioconversion of Fatty Acids into C8–C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1021/acs.jafc.7b05341 Zhu-Jun Zhu 1, 2 , Hai-Min Chen 1 , Juan-Juan Chen 1 , Rui Yang 1 , Xiao-Jun Yan 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1021/acs.jafc.7b05341 Zhu-Jun Zhu 1, 2 , Hai-Min Chen 1 , Juan-Juan Chen 1 , Rui Yang 1 , Xiao-Jun Yan 1
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The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg·L–1 yield. PhLOX could catalyze the one-step bioconversion of C18–C22 fatty acids into C8–C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids. C20:5 was the most suitable substrate among the tested fatty acids. The C8–C9 VOCs were generated in good yields from fatty acids, e.g., 2E-nonenal from C20:4, and 2E,6Z-nonadienal from C20:5. Hydrolyzed oils were also tested as substrates. The reactions mainly generated 2E,4E-pentadienal, 2E-octenal, and 2E,4E-octadienal from hydrolyzed sunflower seed oil, corn oil, and fish oil, respectively. PhLOX showed good stability after storage at 4 °C for 2 weeks and broad tolerance to pH and temperature. These desirable properties of PhLOX make it a promising novel biocatalyst for the industrial production of volatile aroma compounds.
中文翻译：
通过从海坦拟南芥中克隆出的多功能脂氧合酶将脂肪酸一步生物转化为C8–C9挥发性香气化合物
克隆的海坦拟南芥多功能脂氧合酶PhLOX在大肠杆菌中表达，收率为24.4 mg·L –1。PhLOX可以催化将C18–C22脂肪酸一步生物转化为C8–C9挥发性有机化合物（VOC），与二十碳烯酸和二十碳烯酸相比，其显示出更高的催化效率。在测试的脂肪酸中，C20：5是最合适的底物。的C8-C9的VOC是在良好的产率从脂肪酸，例如，2产生ë 4，和2：从C20 -nonenal ë，6 ž从C20 -壬二烯醛：5。还测试了水解油作为底物。反应主要产生2 E，4 E-戊二烯醛，2 E-辛烯醛和2 E，4 E-辛二烯醛分别来自水解的葵花籽油，玉米油和鱼油。PhLOX在4°C下储存2周后显示出良好的稳定性，并且对pH和温度具有宽泛的耐受性。PhLOX的这些理想特性使其成为工业上生产挥发性香气化合物的有前途的新型生物催化剂。
更新日期：2018-01-24
中文翻译：
通过从海坦拟南芥中克隆出的多功能脂氧合酶将脂肪酸一步生物转化为C8–C9挥发性香气化合物
克隆的海坦拟南芥多功能脂氧合酶PhLOX在大肠杆菌中表达，收率为24.4 mg·L –1。PhLOX可以催化将C18–C22脂肪酸一步生物转化为C8–C9挥发性有机化合物（VOC），与二十碳烯酸和二十碳烯酸相比，其显示出更高的催化效率。在测试的脂肪酸中，C20：5是最合适的底物。的C8-C9的VOC是在良好的产率从脂肪酸，例如，2产生ë 4，和2：从C20 -nonenal ë，6 ž从C20 -壬二烯醛：5。还测试了水解油作为底物。反应主要产生2 E，4 E-戊二烯醛，2 E-辛烯醛和2 E，4 E-辛二烯醛分别来自水解的葵花籽油，玉米油和鱼油。PhLOX在4°C下储存2周后显示出良好的稳定性，并且对pH和温度具有宽泛的耐受性。PhLOX的这些理想特性使其成为工业上生产挥发性香气化合物的有前途的新型生物催化剂。
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