In this study, simultaneous carbon oxidation, partial nitritation, denitritation and anammox (SCONDA) was successfully integrated into a one-stage sequencing biofilm batch reactor for treating ammonia-rich organic wastewater with carbon to nitrogen (C/N) ratio of 3. The results showed that 94.3% of COD removal together with 92.6% NH₄⁺-N and 88% TN removal were achieved via SCONDA. High-throughout sequencing analysis further revealed that the microbial community developed in the proposed system was primarily dominated by heterotrophic bacteria (e.g. Thauera, Azovibrio, Ohtaekwangia, Azospira), autotrophic bacteria (e.g. Nitrosomona) and unclassified genus of anammox bacterium, which were all essential for COD and N removal via SCONDA. The observed spatial distributions of the functional species in stratified biofilms were found to be crucial for successful SCONDA at the low dissolved oxygen of 1.3 mg/L. The integrated SCONDA system is expected to offer a promising alternative for advanced nitrogen and organic removal from high-ammonia organic wastewater.

在这项研究中，同时碳氧化，部分硝化，反硝化和厌氧氨氧化（SCONDA）成功地集成到了一步法生物膜间歇式反应器中，以处理碳氮比（C / N）为3的富氨有机废水。结果表明，通过SCONDA可以实现94.3％的COD去除率，92.6％的NH ₄ ⁺ -N和88％TN去除率。高通量测序分析进一步表明，在拟议系统中开发的微生物群落主要由异养细菌（例如Thauera，Azovibrio，Ohtaewangwangia，Azospira），自养细菌（例如Nitrosomona）主导。）和厌氧细菌的未分类属，这对于通过SCONDA去除COD和N都是必不可少的。发现在分层生物膜中观察到的功能物种的空间分布对于在1.3 mg / L的低溶解氧下成功实现SCONDA至关重要。集成的SCONDA系统有望为从高氨有机废水中高级脱除氮和有机物提供有希望的替代方法。