Peptides with molecular weights below 3 kDa from the hydrolysis of olive seed proteins with Thermolysin (OS-3 kDa) have demonstrated a high in vitro hypotensive effect. This fraction has been further fractionated by semipreparative RP-HPLC to obtain 8 fractions. ACE inhibitor capacity of fractions was evaluated observing the highest capacity in fraction F5. Peptides in fraction F5 were identified by RP-HPLC- and HILIC-ESI-Q-ToF and cytotoxic effect was assessed in different cell lines. Peptide LLPSY, present in this faction, was synthesized and characterized. Despite antihypertensive capacity was not as high as in fraction F5, a significant anti-proliferative capacity on two different cancer cell lines was observed. Additional studies to assess antitumor activity confirmed that this peptide showed capability to increase the adhesion capacity of tumor cells, to decrease the migration capacity of cancer cells, and to arrest cell cycle on S phase.

橄榄种子蛋白经嗜热菌素（OS-3 kDa）水解后分子量低于3 kDa的肽已显示出较高的体外降压作用。通过半制备性RP-HPLC进一步分离该级分以获得8个级分。评估馏分的ACE抑制剂容量，并观察到馏分F5的最高容量。通过RP-HPLC-和HILIC-ESI-Q-ToF鉴定级分F5中的肽，并在不同细胞系中评估细胞毒性作用。合成并表征了存在于该分子中的肽LLPSY。尽管抗高血压能力不如F5级，但在两种不同的癌细胞系上却观察到了显着的抗增殖能力。评估抗肿瘤活性的其他研究证实，该肽具有增加肿瘤细胞粘附能力，降低癌细胞迁移能力以及阻止S期细胞周期的能力。