In the present study, antioxidant fraction (C2) has been obtained from a hazelnut processing by-product. The C2 exhibited strong antioxidant and cytoprotective effect against Ang II induced oxidant injury via up-regulation of SOD and heme oxygenase-1 (HO-1) levels and down-regulation of xanthine oxidase-1 (XO-1) level to inhibit the production of ROS synthesis in human umbilical vein endothelial cells (HUVECs). LC-ESI-MS/MS results revealed that C2 was found to include six novel peptides and their amino acid sequences were ADGF (408.16 Da), AGGF (350.16 Da), AWDPE (616.25 Da), DWDPK (659.29 Da), ETTL (462.23 Da), and SGAF (380.17 Da). All six synthetic peptides showed excellent antioxidant activity. Especially, the ADGF peptide exhibited the highest ABTS radical scavenging activity and DPPH radical scavenging ability among them. Therefore, the peptides isolated from wild hazelnut protein hydrolysates may be used as potential antioxidants in the food and drug industries.

在本研究中，已从榛子加工副产物中获得了抗氧化剂组分（C2）。C2通过上调SOD和血红素加氧酶-1（HO-1）的水平并下调黄嘌呤氧化酶-1（XO-1）的水平来抑制Ang II诱导的Ang损伤，从而表现出强大的抗氧化和细胞保护作用。脐静脉内皮细胞（HUVEC）中ROS合成的研究 LC-ESI-MS / MS结果显示，C2被发现包括六个新肽，其氨基酸序列分别为ADGF（408.16 Da），AGGF（350.16 Da），AWDPE（616.25 Da），DWDPK（659.29 Da），ETTL（ 462.23 Da）和SGAF（380.17 Da）。所有六个合成肽均显示出优异的抗氧化活性。特别地，ADGF肽在其中表现出最高的ABTS自由基清除活性和DPPH自由基清除能力。所以，