A QuEChERS extraction method followed by HPLC-MS/MS analysis was developed to simultaneously analyze chlordecone and its metabolite chlordecol in animal livers. The overall method was validated with accuracy profiles according to the French Standard NF V03-110 and European Union guidelines. The validation was performed on bovine, ovine and porcine liver samples. Linearity, matrix effect, accuracy, within-laboratory repeatability, specificity, LOQ, Q/q relative ion intensities, and uncertainty were reported. Recoveries were between 70% and 120%. LOQs of 1.36 µg chlordecone kg⁻¹ and 2.50 µg chlordecol kg⁻¹ of fresh liver were found. Twelve contaminated livers of bovine, ovine and porcine origin from the French West Indies or samples from in vivo studies were analyzed. In these liver samples from contaminated animals, chlordecone was quantified at concentrations higher than the maximum residue limit and chlordecol in very low amounts in all the samples. In addition, these results confirm that chlordecone can be metabolized in ruminant species.

开发了一种QuEChERS提取方法，然后进行HPLC-MS / MS分析，以同时分析动物肝脏中的十氯酮及其代谢产物十氢萘酚。根据法国标准NF V03-110和欧盟准则，使用精度曲线对整个方法进行了验证。验证是在牛，羊和猪肝样品上进行的。报告了线性，基质效应，准确性，实验室内重复性，特异性，LOQ，Q / q相对离子强度和不确定度。回收率在70％至120％之间。发现新鲜肝脏的定量限为1.36 µg十氯酮kg ^-1和2.50 µg十氯酮kg ^-1。来自法属西印度群岛的十二种受污染的牛，羊和猪肝脏或体内样品研究进行了分析。在这些来自受污染动物的肝脏样品中，十氯酮的浓度要高于最大残留限量，而所有样品中的十氯酚含量都非常低。此外，这些结果证实了十氯酮可以在反刍动物中代谢。