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Investigation into allergenicity reduction and glycation sites of glycated β-lactoglobulin with ultrasound pretreatment by high-resolution mass spectrometry
Food Chemistry ( IF 8.5 ) Pub Date : 2018-01-12 , DOI: 10.1016/j.foodchem.2018.01.086
Guang-xian Liu , Zong-cai Tu , Wenhua Yang , Hui Wang , Lu Zhang , Da Ma , Tao Huang , Jun Liu , Xue Li

Ultrasound treatment could change the conformation of β-lactoglobulin (β-Lg) and improve the glycation reaction in aqueous solution under neutral condition. However, the effect of ultrasound pretreatment on glycation of β-Lg with pentose at dry-state remains ambiguous, and the relationship between glycation and allergenicity of β-Lg with ultrasound pretreatment is unclear. This study aimed to evaluate the effect of ultrasound pretreatment on glycation and allergenicity of β-Lg. Markedly decreased allergenicity of β-Lg was observed after glycation with ribose before and after ultrasound pretreatment with the minimum found at 400 W. Orbitrap LC–MS/MS showed that the glycation degree of some peptides in glycated β-Lg with and without ultrasound pretreatment were different although the content of free amino group and molecular mass were insignificantly different. Therefore, ultrasound pretreatment promoted the reduction in allergenicity by improving the glycation extent of some glycation sites although it hardly enhanced the whole glycation degree of β-Lg.



中文翻译:

超声预处理超声预处理糖基化β-乳球蛋白的致敏性降低和糖基化位点的研究

超声处理可改变β-乳球蛋白(β- Lg)的构象,并改善中性条件下水溶液中的糖基化反应。然而,在糖化超声预处理效果β与在干燥状态的戊糖-Lg保持模糊,糖化和变应原性的关系β与超声预处理-Lg还不清楚。本研究旨在评估超声预处理对β- Lg糖基化和变应原性的影响。β的致敏性明显降低超声预处理前后用核糖糖化后可观察到-Lg,最小值为400W。Orbitrap LC-MS / MS表明,经糖化的β- Lg中某些肽的糖化程度(经超声预处理和未经超声预处理)有所不同,尽管其含量游离氨基的分子量和分子量无明显差异。因此,尽管超声波预处理几乎不提高β- Lg的整体糖化度,但通过改善某些糖化位点的糖化程度促进了变应原性的降低。

更新日期:2018-01-12
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