The antioxidant activity and genotoxicity of isogarcinol were assessed by several in vitro tests. Its IC₅₀ values for DPPH and ABTS were 36.3 ± 3.35 µM and 16.6 ± 3.98 µM, respectively, which were all lower than those of VC and BHT. Isogarcinol had no cyctotoxic or promotional activities at 1–10 µM in the CCK-8 assay, and negligible genotoxic effects at 50–500 µM on HepG2 cells by the single-cell gel electrophoresis assay. Pre-incubation of the cells with 0.5–1.5 µM isogarcinol, before exposure to H₂O₂, significantly increased cell viability in a concentration-dependent manner. Isogarcinol also reduced intercellular reactive oxygen species accumulation, lactate dehydrogenase release and malondialdehyde levels, and increased superoxide dismutase activity and glutathione levels. Western-blot analysis revealed that it up-regulated pro-caspase-3 and reduced cleaved caspase-3 during H₂O₂-induced oxidative stress. All the above results indicate that isogarcinol promises to be useful as a natural antioxidant.

通过几种体外试验评估了异藤黄素的抗氧化活性和遗传毒性。DPPH和ABTS的IC ₅₀值分别为36.3±3.35 µM和16.6±3.98 µM，均低于VC和BHT。在CCK-8分析中，异黄霉素在1–10 µM时没有细胞毒性或促进活性，而在单细胞凝胶电泳分析中，在50–500 µM时对HepG2细胞的遗传毒性作用微不足道。在暴露于H ₂ O ₂之前，将细胞与0.5–1.5 µM异藤黄素一起预孵育，以浓度依赖的方式显着增加了细胞活力。异丁香酚还减少了细胞间活性氧的积累，乳酸脱氢酶的释放和丙二醛的水平，并增加了超氧化物歧化酶的活性和谷胱甘肽的水平。Western印迹分析表明，它在H ₂ O ₂诱导的氧化应激过程中上调了半胱天冬酶原3，并减少了切割的半胱天冬酶3 。以上所有结果表明，异藤黄酚有望用作天然抗氧化剂。