To identify food and beverages that provide the regular intake of natural compounds capable of interfering with toxic amyloidogenic aggregates, we developed an experimental protocol that combines NMR spectroscopy and atomic force microscopy, in vitro biochemical and cell assays to detect anti-Aβ molecules in natural edible matrices.

We applied this approach to investigate the potential anti-amyloidogenic properties of coffee and its molecular constituents.

Our data showed that green and roasted coffee extracts and their main components, 5-O-caffeoylquinic acid and melanoidins, can hinder Aβ on-pathway aggregation and toxicity in a human neuroblastoma SH-SY5Y cell line. Coffee extracts and melanoidins also counteract hydrogen peroxide- and rotenone-induced cytotoxicity and modulate some autophagic pathways in the same cell line.

为了确定可正常摄入能够干扰有毒淀粉样蛋白聚集体的天然化合物的食品和饮料，我们开发了一种实验方案，该方案将NMR光谱和原子力显微镜，体外生化和细胞分析相结合，以检测天然食用食品中的抗Aβ分子矩阵。

我们应用这种方法来调查咖啡及其分子成分的潜在抗淀粉样变性特性。

我们的数据表明，绿色和烘焙过的咖啡提取物及其主要成分5- O-咖啡酰奎尼酸和黑色素可阻碍Aβ在人成神经细胞瘤SH-SY5Y细胞系中的聚集和毒性。咖啡提取物和类黑素还可以抵消过氧化氢和鱼藤酮诱导的细胞毒性，并调节同一细胞系中的某些自噬途径。