Pleurotus sajor-caju (PSC) is an edible mushroom used in food supplements, presenting antitumor properties through induction of cell death pathways. The PSC potential against colorectal cancer was analyzed by exposing HCT116^wt cells to different PSC extracts. The PSC n-hexane extract (PSC-hex) showed the highest cytotoxicity effect (IC₅₀ value 0.05 mg/mL). The observed cytotoxicity was then associated to apoptosis-promoting and cell cycle-arrest pathways. PSC-hex was able to induce apoptosis related to breakdown of mitochondrial membrane potential and ROS generation. The absence of cytotoxicity in HTC116^-p53 and HTC116^-Bax cells, alongside with an increase in p53, Bax and Caspase-3 expression, and decrease in Bcl-2 expression, supports that the pro-apoptotic effect is probably induced through a p53 associated pathway. PSC-hex induced cell cycle arrest at G2/M in HCT116^wt without cytotoxicity in HTC116^-p21 cells. These findings suggest that a p21/p53 cell cycle regulation pathway is probably disrupted by compounds present on PSC-hex. Identification of the major components was then performed with ergosta-5,7,22-trien-3β-ol representing 30.6% of total weight. In silico docking studies of ergosta-5,7,22-trien-3β against Bcl-2 were performed and results show a credible interaction with the Bcl-2 hydrophobic cleft. The results show that PSC-hex can be used as supplementary food for adjuvant therapy in colorectal carcinoma.

杏鲍菇（PSC）是一种食用蘑菇，用于食品补充剂，通过诱导细胞死亡途径表现出抗肿瘤特性。通过将HCT116 ^wt细胞暴露于不同的PSC提取物中来分析针对大肠癌的PSC潜力。PSC正己烷提取物（PSC-hex）表现出最高的细胞毒性作用（IC ₅₀值为0.05 mg / mL）。然后，观察到的细胞毒性与促进细胞凋亡和阻滞细胞周期的途径有关。PSC-hex能够诱导与线粒体膜电位破坏和ROS产生有关的凋亡。HTC116 ^-p53和HTC116 ^-Bax中没有细胞毒性细胞，以及p53，Bax和Caspase-3表达的增加以及Bcl-2表达的减少，都支持促凋亡作用可能是通过p53相关途径诱导的。PSC-六角诱导的细胞周期停滞在G2 / M在HCT116^与wt无细胞毒性在HTC116 ^-p21 细胞。这些发现表明，p21 / p53细胞周期调控途径可能被PSC-hex上存在的化合物破坏。然后，使用占总重量30.6％的ergosta-5,7,22-trien-3β-ol进行主要成分的鉴定。在计算机对接研究中，ergosta-5、7、22-trien-3β与Bcl-2发生了对接，结果表明与Bcl-2疏水裂缝的相互作用是可信的。结果表明，PSC-hex可作为大肠癌辅助治疗的辅助食品。